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莱茵衣藻经甲苯处理的细胞中叶绿体核糖核酸的合成

Synthesis of chloroplast ribonucleic acid in Chlamydomonas reinhardtii toluene-treated cells.

作者信息

Guertin M, Bellemare G

出版信息

Eur J Biochem. 1979 May 2;96(1):125-9. doi: 10.1111/j.1432-1033.1979.tb13021.x.

DOI:10.1111/j.1432-1033.1979.tb13021.x
PMID:256550
Abstract

Chlamydomonas reinhardtii cells treated with toluene at 0 degrees C and 25 degrees C incorporate ribonucleoside triphosphates (NTPs) into chloroplast RNA at 25 degrees C and also at 35 degrees C. The incorporation requires all four NTPs and Mg2+, and is completely inhibited by DNase, RNase, actinomycin D (40 microgram/ml) and rifampicin (350 microgram/ml). However, the incorporation is almost totally insensitive to both alpha-amanitin and streptolydigin at 200 microgram/ml.

摘要

在0摄氏度和25摄氏度下用甲苯处理的莱茵衣藻细胞，在25摄氏度以及35摄氏度时会将核糖核苷三磷酸（NTPs）掺入叶绿体RNA中。这种掺入需要所有四种NTPs和Mg2+，并且会被DNase、RNase、放线菌素D（40微克/毫升）和利福平（350微克/毫升）完全抑制。然而，在200微克/毫升时，这种掺入对α-鹅膏蕈碱和利链菌素几乎完全不敏感。

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