Howell S H, Walker L L
Proc Natl Acad Sci U S A. 1972 Feb;69(2):490-4. doi: 10.1073/pnas.69.2.490.
Toluene-treated Chlamydomonas reinhardi incorporate deoxynucleoside triphosphates (dNTPs) into DNA. Incorporation requires all four dNTPs, but requires neither ATP nor added DNA. The incorporation reaction is linear for nearly 20 min. The product of synthesis in treated cells is mainly chloroplast (beta-component) DNA. However, a small quantity of nuclear (alpha-component) DNA is also synthesized. Synchronously grown cells are most efficient in dNTP incorporation at a time in the cell cycle when chloroplast DNA is normally replicated. Toluene treatment disrupts the internal morphology of the cell, but leaves the outer membrane of the chloroplast intact.
经甲苯处理的莱茵衣藻能将脱氧核苷三磷酸（dNTPs）掺入DNA。掺入反应需要所有四种dNTPs，但既不需要ATP也不需要添加DNA。掺入反应在近20分钟内呈线性。处理后细胞中的合成产物主要是叶绿体（β组分）DNA。然而，也会合成少量的核（α组分）DNA。同步生长的细胞在细胞周期中叶绿体DNA正常复制的某个时间点，掺入dNTPs的效率最高。甲苯处理会破坏细胞的内部形态，但叶绿体的外膜保持完整。
