Gawande S J, Gurav V S, Ingle A A, Martin D P, Asokan R, Gopal J
ICAR-Directorate of Onion and Garlic Research, Rajgurunagar, Pune, 410505, India,
Arch Virol. 2015 May;160(5):1285-9. doi: 10.1007/s00705-015-2354-x. Epub 2015 Feb 6.
The nucleotide sequence of M- and S-RNA segments of an Indian iris yellow spot virus (IYSV) were determined. Sequence comparisons showed that both of these sequences shared less than 95 % identity with those other known IYSV isolates. Phylogenetic analysis revealed that the S- and M-RNA sequences of known IYSV isolates clustered with those of the tospoviruses, tomato yellow ring virus, polygonum ringspot virus and hippeastrum chlorotic ringspot virus. Further, multiple recombination detection methods detected inter- and intra-species recombination events that clustered primarily within the intergenic regions of S- and M-RNA, suggesting that these are possibly recombination hotspots in IYSV and closely related tospoviruses.
测定了一种印度鸢尾黄斑病毒（IYSV）的M和S RNA片段的核苷酸序列。序列比较表明，这两个序列与其他已知的IYSV分离株的序列同一性均低于95%。系统发育分析显示，已知IYSV分离株的S和M RNA序列与番茄黄环病毒、虎杖环斑病毒和朱顶红褪绿环斑病毒等番茄斑萎病毒属病毒的序列聚类在一起。此外，多种重组检测方法检测到种间和种内重组事件，这些事件主要聚集在S和M RNA的基因间隔区内，表明这些区域可能是IYSV和密切相关的番茄斑萎病毒属病毒的重组热点。
