Instituto de Biología Integrativa de Sistemas (I2SysBio), Consejo Superior de Investigaciones Científicas-Universitat de València, 46182, Paterna, Spain.
The Santa Fe Institute, Santa Fe, NM, 87501, USA.
Arch Virol. 2021 Feb;166(2):491-499. doi: 10.1007/s00705-020-04902-1. Epub 2021 Jan 4.
The family Tospoviridae of the order Bunyavirales is constituted of tri-segmented negative-sense single-stranded RNA viruses that infect plants and are also able to replicate in their insect vectors in a persistent manner. The family is composed of a single genus, Orthotospovirus, whose type species is Tomato spotted wilt orthotospovirus. Previous studies assessing the phylogenetic relationships within this genus were based on partial genomic sequences, resulting in unresolved clades and a poor assessment of the roles of recombination and segment reassortment during mixed infections. Full genome sequences of members of recognized Orthotospovirus species are now available at NCBI. In this study, we examined 67 complete genome sequences from members of 22 species. Our study confirms the existence of four phylogroups (A to D), grouped in two major clades (A-B and C-D) within the genus. We found strong evidence that within-segment recombination events and reassortment of segments during mixed infections have been involved in the origin of new orthotospoviruses. Also, selection pressures were analyzed for each gene, and evidence of positive selection was found in all genes.
弹状病毒目（Bunyavirales）的 Tospoviridae 科由感染植物的三片段负义单链 RNA 病毒组成，并且能够以持续的方式在其昆虫载体中复制。该科由一个单一的属，Orthotospovirus 组成，其模式种是番茄斑萎病毒。以前评估该属内系统发育关系的研究是基于部分基因组序列，导致未解决的进化枝和对重组和片段重配在混合感染期间的作用的评估不佳。在 NCBI 现在可以获得公认的 Orthotospovirus 物种的成员的全基因组序列。在这项研究中，我们检查了来自 22 个物种的 67 个完整基因组序列。我们的研究证实了四个谱系（A 到 D）的存在，在该属内分为两个主要进化枝（A-B 和 C-D）。我们发现有强有力的证据表明，在混合感染期间的片段内重组事件和片段重配参与了新的 orthotospovirus 的起源。此外，还对每个基因进行了选择压力分析，并在所有基因中发现了正选择的证据。
