Padiernos Ryan Bismark C, Balbin Michelle M, Parayao Arman M, Mingala Claro N
Animal Health Unit, Philippine Carabao Center National Headquarters and Gene Pool, Science City of Munoz, 3120, Nueva Ecija, Philippines.
Arch Virol. 2015 Apr;160(4):969-78. doi: 10.1007/s00705-015-2359-5. Epub 2015 Feb 6.
Caprine arthritis encephalitis virus (CAEV) causes caprine arthritis encephalitis syndrome, which is an emerging disease of goats in the Philippines. DNA sequence analysis showed homology of 86-93 % between Philippine CAEV and available CAEV sequences in GenBank. CAEV was detected using nested polymerase chain reaction (PCR), and new sets of primers were designed in order to amplify the gag gene, which is a highly conserved region of the viral genome. In addition, the Philippine CAEV isolate clustered in group B with the prototype caprine lentivirus. Based on amino acid sequence alignments, it is possible that the Philippine CAEV isolate is a new strain of CAEV, but it is also possible that it was already present in the country even before the start of goat importation. Molecular characterization of the CAEV gag gene is important for the development of a detection kit specific for the local strain of CAEV and the establishment of small ruminant lentivirus eradication programs in the Philippines. This study is the first report to describe the molecular characteristics of CAEV circulating in the Philippines.
山羊关节炎脑炎病毒（CAEV）可引发山羊关节炎脑炎综合征，这是菲律宾山羊中一种新出现的疾病。DNA序列分析显示，菲律宾CAEV与GenBank中现有CAEV序列的同源性为86%至93%。使用巢式聚合酶链反应（PCR）检测CAEV，并设计了新的引物组以扩增gag基因，该基因是病毒基因组的一个高度保守区域。此外，菲律宾CAEV分离株与山羊慢病毒原型聚集在B组。基于氨基酸序列比对，菲律宾CAEV分离株有可能是CAEV的一个新菌株，但也有可能在该国山羊进口开始之前就已存在。CAEV gag基因的分子特征对于开发针对当地CAEV菌株的检测试剂盒以及在菲律宾建立小型反刍动物慢病毒根除计划具有重要意义。本研究是首篇描述在菲律宾传播的CAEV分子特征的报告。
