School of Civil, Environmental and Architectural Engineering, Korea University, Seoul, South Korea.
School of Civil, Environmental and Architectural Engineering, Korea University, Seoul, South Korea.
Water Res. 2015 Apr 15;73:132-44. doi: 10.1016/j.watres.2015.01.014. Epub 2015 Jan 22.
Continuously feeding influent wastewater containing diverse bacterial species to a wastewater treatment activated sludge bioreactor may influence the activated sludge bacterial community temporal dynamics. To explore this possibility, this study tracked influent wastewater and activated sludge bacterial communities by pyrosequencing 16S rRNA genes from four full-scale wastewater treatment plants over a 9-month period. The activated sludge communities showed significantly higher richness and evenness than the influent wastewater communities. Furthermore, the two communities were different in composition and temporal dynamics. These results demonstrate that the impact of the influent wastewater communities on the activated sludge communities was weak. Nevertheless, 4.3-9.3% of the operational taxonomic units (OTUs) detected in the activated sludge were shared with the influent wastewater, implying contribution from influent wastewater communities to some extent. However, the relative OTU abundance of the influent wastewater was not maintained in the activated sludge communities (i.e., weak neutral assembly). In addition, the variability of the communities of the shared OTUs was moderately correlated with abiotic factors imposed to the bioreactors. Taken together, temporal dynamics of activated sludge communities appear to be predominantly explained by species sorting processes in response to influent wastewater communities.
连续向废水处理活性污泥生物反应器中添加含有多种细菌物种的废水可能会影响活性污泥细菌群落的时间动态。为了探索这种可能性，本研究通过对四个全规模废水处理厂的废水和活性污泥中的 16S rRNA 基因进行焦磷酸测序，在 9 个月的时间内跟踪了进水和活性污泥细菌群落。活性污泥群落的丰富度和均匀度明显高于进水群落。此外，这两个群落的组成和时间动态也不同。这些结果表明，进水群落对活性污泥群落的影响较弱。尽管如此，在活性污泥中检测到的 4.3%-9.3%的操作分类单元（OTUs）与进水共享，这意味着进水群落在一定程度上对活性污泥群落有贡献。然而，进水群落的相对 OTU 丰度并没有在活性污泥群落中得到维持（即弱中性组装）。此外，共享 OTUs 的群落变化与施加给生物反应器的非生物因素中度相关。综上所述，活性污泥群落的时间动态似乎主要是通过对进水群落的物种分选过程来解释的。
