da Silva Marcio A C, Oliveira Renata N, Mendonça Roberta Helena, Lourenço Talita G B, Colombo Ana Paula V, Tanaka Marcelo N, Tude Elena M O, da Costa Marysilvia F, Thiré Rossana Mara S M
COPPE/Program of Metallurgical and Materials Engineering, Federal University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
Chemical Engineering Department of the Technology Institute, Federal Rural University of Rio de Janeiro, Rio de Janeiro City, Rio de Janeiro, Brazil.
J Biomed Mater Res B Appl Biomater. 2016 Jan;104(1):106-15. doi: 10.1002/jbm.b.33357. Epub 2015 Feb 6.
Guided tissue regeneration is a technique used for periodontium reconstruction. This technique uses barrier membranes, which prevent epithelial growth in the wound site and may also be used to release antibiotics, to protect the wound against opportunistic infections. Periodontal poly(3-hydroxybutyrate) membranes containing metronidazole (a drug used to help in infection control) were produced and characterized. The kinetic mechanism of the metronidazole delivery of leached and nonleached membrane as well as its cytotoxicity and structural integrity were evaluated. Poly(3-hydroxybutyrate) membranes containing 0.5-2 wt % of the drug and 20 wt % of the plasticizer were manufactured via compression molding. Based on morphological analysis, membranes loaded with 2% metronidazole were considered for detailed studies. The results revealed that metronidazole delivery by the leached membranes seemed to follow the Fick's law. Membranes were noncytotoxic. The amount of metronidazole delivered was in the range of the minimal inhibitory concentration for Porphyromonas gingivalis, and the membranes inhibited the proliferation of these bacteria. Besides, they maintained their mechanical resistance after 30 days of immersion in phosphate buffer at pH 7.4.
引导组织再生是一种用于牙周组织重建的技术。该技术使用屏障膜，其可防止伤口部位上皮生长，还可用于释放抗生素，以保护伤口免受机会性感染。制备并表征了含有甲硝唑（一种用于控制感染的药物）的牙周聚（3-羟基丁酸酯）膜。评估了浸出和未浸出膜中甲硝唑释放的动力学机制及其细胞毒性和结构完整性。通过压缩成型制备了含有0.5-2 wt%药物和20 wt%增塑剂的聚（3-羟基丁酸酯）膜。基于形态分析，选择负载2%甲硝唑的膜进行详细研究。结果表明，浸出膜中甲硝唑的释放似乎遵循菲克定律。膜无细胞毒性。甲硝唑的释放量在牙龈卟啉单胞菌最小抑菌浓度范围内，且该膜抑制了这些细菌的增殖。此外，它们在pH 7.4的磷酸盐缓冲液中浸泡30天后仍保持机械抗性。
