Department of Chemistry, Dankook University, 126 Jukjeon-dong, Yongin-si, Gyeonggi-do 448-701 (Korea).
Angew Chem Int Ed Engl. 2015 Mar 23;54(13):3912-6. doi: 10.1002/anie.201410548. Epub 2015 Feb 4.
A family of organic chromophores that, like pyrene, forms emissive excimers is reported. Their chemical and photophysical properties are superior to pyrene for the design of chemo- and biosensors. Unlike hydrophobic pyrene, which requires excitation by cell-damaging UV irradiation, these polar dyes absorb strongly in the visible range, and their excimers emit brightly in the red to far-red region of the electromagnetic spectrum. The intensity of the emission signal is greatly increased upon formation of a preassociated dimer that is triggered upon aggregation or crystallization. In demonstration of the potential of this new family of excimer-forming dyes, a probe that is capable of detecting label-free DNA in water down to 10 pM and also doubles as a visualization agent for DNA in gel electrophoresis is reported.
报道了一类与芘类似的有机生色团家族，它们能够形成发射激基缔合物。与需要细胞损伤性紫外光激发的疏水性芘相比，这类极性染料在可见光范围内具有较强的吸收，其激基缔合物在电磁光谱的红到远红区域中发出明亮的光。在聚集或结晶时，预缔合二聚体形成，其强烈增加了发射信号的强度。作为这种新型激基缔合物形成染料家族的潜力的证明，报道了一种能够在水中检测到低至 10 pM 的无标记 DNA 的探针，并且还可以作为凝胶电泳中 DNA 的可视化试剂。
