Kim Eun T, Guan Le Luo, Lee Shin J, Lee Sang M, Lee Sang S, Lee Il D, Lee Su K, Lee Sung S
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta T6G 2R3, Canada .
Division of Applied Life Science (BK21 , IALS), Gyeongsang National University, Jinju 660-701, Korea .
Asian-Australas J Anim Sci. 2015 Apr;28(4):530-7. doi: 10.5713/ajas.14.0692.
The objective of this study was to evaluate the in vitro effects of flavonoid-rich plant extracts (PE) on ruminal fermentation characteristics and methane emission by studying their effectiveness for methanogenesis in the rumen. A fistulated Holstein cow was used as a donor of rumen fluid. The PE (Punica granatum, Betula schmidtii, Ginkgo biloba, Camellia japonica, and Cudrania tricuspidata) known to have high concentrations of flavonoid were added to an in vitro fermentation incubated with rumen fluid. Total gas production and microbial growth with all PE was higher than that of the control at 24 h incubation, while the methane emission was significantly lower (p<0.05) than that of the control. The decrease in methane accumulation relative to the control was 47.6%, 39.6%, 46.7%, 47.9%, and 48.8% for Punica, Betula, Ginkgo, Camellia, and Cudrania treatments, respectively. Ciliate populations were reduced by more than 60% in flavonoid-rich PE treatments. The Fibrobacter succinogenes diversity in all added flavonoid-rich PE was shown to increase, while the Ruminoccocus albus and R. flavefaciens populations in all PE decreased as compared with the control. In particular, the F. succinogenes community with the addition of Birch extract increased to a greater extent than that of others. In conclusion, the results of this study showed that flavonoid-rich PE decreased ruminal methane emission without adversely affecting ruminal fermentation characteristics in vitro in 24 h incubation time, suggesting that the flavonoid-rich PE have potential possibility as bio-active regulator for ruminants.
本研究的目的是通过研究富含黄酮类化合物的植物提取物（PE）对瘤胃甲烷生成的有效性，来评估其对瘤胃发酵特性和甲烷排放的体外影响。选用一头装有瘤胃瘘管的荷斯坦奶牛作为瘤胃液供体。将已知富含黄酮类化合物的PE（石榴、岳桦、银杏、山茶和柘树）添加到与瘤胃液一起进行的体外发酵体系中。在24小时培养时，所有PE组的总产气量和微生物生长均高于对照组，而甲烷排放量显著低于对照组（p<0.05）。与对照组相比，石榴、岳桦、银杏、山茶和柘树处理组的甲烷积累量分别减少了47.6%、39.6%、46.7%、47.9%和48.8%。在富含黄酮类化合物的PE处理组中，纤毛虫数量减少了60%以上。所有添加富含黄酮类化合物的PE组中，琥珀酸丝状杆菌的多样性均增加，而与对照组相比，所有PE组中的白色瘤胃球菌和黄色瘤胃球菌数量均减少。特别是，添加桦树提取物后，琥珀酸丝状杆菌群落的增加幅度比其他组更大。总之，本研究结果表明，在24小时培养时间内，富含黄酮类化合物的PE可降低瘤胃甲烷排放，且不会对瘤胃体外发酵特性产生不利影响，这表明富含黄酮类化合物的PE有作为反刍动物生物活性调节剂的潜在可能性。
