Tropical Feed Resources Research and Development Center (TROFREC), Department of Animal Science, Faculty of Agriculture, Khon Kaen University, Khon Kaen, 40002, Thailand.
Division of Animal Science, Faculty of Agricultural Technology, Rajamangala University of Technology Thanyaburi, Thanyaburi, 12130, Pathum Thani, Thailand.
Sci Rep. 2024 Jun 23;14(1):14425. doi: 10.1038/s41598-024-59697-x.
The objective of this study was to investigate the effect of microencapsulated bioactive compounds from lemongrass mixed dragon fruit peel pellet (MiEn-LEDRAGON) supplementation on fermentation characteristics, nutrient degradability, methane production, and the microbial diversity using in vitro gas production technique. The study was carried out using a completely randomized design (CRD) with five levels of MiEn-LEDRAGON supplementation at 0, 1, 2, 3, and 4% of the total dry matter (DM) substrate. Supplementation of MiEn-LEDRAGON in the diet at levels of 3 or 4% DM resulted in increased (p < 0.05) cumulative gas production at 96 hours (h) of incubation time, reaching up to 84.842 ml/ 0.5 g DM. Furthermore, supplementation with 3% MiEn-LEDRAGON resulted in higher in vitro nutrient degradability and ammonia-nitrogen concentration at 24 h of the incubation time when compared to the control group (without supplementation) by 5.401% and 11.268%, respectively (p < 0.05). Additionally, supplementation with MiEn-LEDRAGON in the diet led to an increase in the population of Fibrobacter succinogenes at 24 h and Butyrivibrio fibrisolvens at 12 h, while decreasing the population of Ruminococcus albus, Ruminococcus flavefaciens, and Methanobacteriales (p < 0.05). Moreover, supplementation of MiEn-LEDRAGON in the diet at levels of 2 to 4% DM resulted in a higher total volatile fatty acids (VFA) at 24 h, reaching up to 73.021 mmol/L (p < 0.05). Additionally, there was an increased proportion of propionic acid (C3) and butyric acid (C4) at 12 h (p < 0.05). Simultaneously, there was a decrease in the proportion of acetic acid (C2) and the ratio of acetic acid to propionic acid (C2:C3), along with a reduction of methane (CH) production by 11.694% when comparing to the 0% and 3% MiEn-LEDRAGON supplementation (p < 0.05). In conclusion, this study suggests that supplementing MiEn-LEDRAGON at 3% of total DM substrate could be used as a feed additive rich in phytonutrients for ruminants.
本研究旨在通过体外产气技术，研究添加香茅-火龙果果皮微胶囊化生物活性化合物（MiEn-LEDRAGON）对发酵特性、养分降解率、甲烷产量和微生物多样性的影响。研究采用完全随机设计（CRD），在 0、1、2、3 和 4%总干物质（DM）底物水平下添加五种不同水平的 MiEn-LEDRAGON。在日粮中添加 3 或 4% DM 的 MiEn-LEDRAGON 可增加（p<0.05）96 小时孵育时间的累积产气量，达到 84.842ml/0.5g DM。此外，与对照组（未添加）相比，3% MiEn-LEDRAGON 组在 24 小时的体外养分降解率和氨氮浓度分别提高了 5.401%和 11.268%（p<0.05）。此外，日粮中添加 MiEn-LEDRAGON 可增加 24 小时产琥珀酸丝状杆菌和 12 小时产丁酸梭菌的数量，同时降低白瘤胃球菌、黄瘤胃球菌和甲烷杆菌的数量（p<0.05）。此外，日粮中添加 2%至 4% DM 的 MiEn-LEDRAGON 可提高 24 小时总挥发性脂肪酸（VFA）水平，达到 73.021mmol/L（p<0.05）。此外，12 小时丙酸（C3）和丁酸（C4）的比例增加（p<0.05）。同时，乙酸（C2）的比例和乙酸与丙酸的比例（C2：C3）降低，甲烷（CH）产量降低 11.694%，与 0%和 3% MiEn-LEDRAGON 组相比（p<0.05）。综上所述，本研究表明，在总 DM 底物中添加 3%的 MiEn-LEDRAGON 可用作富含植物营养素的反刍动物饲料添加剂。
