Department of Radiology, Ajou University School of Medicine, Suwon, Korea ; Musculoskeletal Imaging Laboratory, Ajou University Medical Center, Suwon, Korea.
Department of Orthopedic Surgery, Ajou University School of Medicine, Suwon, Korea ; Cartilage Regeneration Center, Ajou University Medical Center, Suwon, Korea.
Ultrasonography. 2015 Apr;34(2):115-24. doi: 10.14366/usg.14047. Epub 2014 Dec 28.
To demonstrate the superficial hyperechoic band (SHEB) in articular cartilage by using ultrasonography (US) and to assess its correlation with histological images.
In total, 47 regions of interest (ROIs) were analyzed from six tibial osteochondral specimens (OCSs) that were obtained after total knee arthroplasty. Ultrasonograms were obtained for each OCS. Then, matching histological sections from all specimens were obtained for comparison with the ultrasonograms. Two types of histological staining were used: Safranin-O stain (SO) to identify glycosaminoglycans (GAG) and Masson's trichrome stain (MT) to identify collagen. In step 1, two observers evaluated whether there was an SHEB in each ROI. In step 2, the two observers evaluated which histological staining method correlated better with the SHEB by using the ImageJ software.
In step 1 of the analysis, 20 out of 47 ROIs showed an SHEB (42.6%, kappa=0.579). Step 2 showed that the SHEB correlated significantly better with the topographical variation in stainability in SO staining, indicating the GAG distribution, than with MT staining, indicating the collagen distribution (P<0.05, kappa=0.722).
The SHEB that is frequently seen in human articular cartilage on high-resolution US correlated better with variations in SO staining than with variations in MT staining. Thus, we suggest that a SHEB is predominantly related to changes in GAG. Identifying an SHEB by US is a promising method for assessing the thickness of articular cartilage or for monitoring early osteoarthritis.
通过超声（US）显示关节软骨的浅层强回声带（SHEB），并评估其与组织学图像的相关性。
共分析了 6 个全膝关节置换术后获得的胫骨骨软骨标本（OCS）的 47 个感兴趣区（ROI）。对每个 OCS 进行超声检查。然后，获取所有标本的匹配组织学切片，与超声图像进行比较。使用两种组织学染色方法：番红 O 染色（SO）识别糖胺聚糖（GAG）和 Masson 三色染色（MT）识别胶原。在步骤 1 中，两位观察者评估每个 ROI 是否存在 SHEB。在步骤 2 中，两位观察者使用 ImageJ 软件评估哪种组织学染色方法与 SHEB 的相关性更好。
在分析的第 1 步中，47 个 ROI 中有 20 个（42.6%，kappa=0.579）显示 SHEB。第 2 步显示，SHEB 与 SO 染色的染色均匀性的地形变化相关性更好，表明 GAG 分布，与 MT 染色相关性较差，表明胶原分布（P<0.05，kappa=0.722）。
在高分辨率 US 中经常在人关节软骨中观察到的 SHEB 与 SO 染色的变化相关性更好，与 MT 染色的变化相关性较差。因此，我们认为 SHEB 主要与 GAG 的变化有关。通过 US 识别 SHEB 是评估关节软骨厚度或监测早期骨关节炎的有前途的方法。
