Roy & Diana Vagelos Laboratories, Department of Chemistry, University of Pennsylvania, Philadelphia, Pennsylvania 19104-6323 (USA) http://percec02.chem.upenn.edu/
Angew Chem Int Ed Engl. 2015 Mar 23;54(13):4036-40. doi: 10.1002/anie.201410882. Epub 2015 Feb 5.
Glycodendrimersomes with programmable surface display of glycan, together with artificially engineered galectins, were used to understand the physiological significance of human lectins with homodimeric and tandem-repeat-type displays. The mode of topological surface presentation and the density of glycan affected vesicle aggregation mediated by multivalent carbohydrate-protein interactions. The cross-linking capacity of homodimeric lectins was enhanced by covalent connection of the two carbohydrate-binding sites. These findings highlight the value of glycodendrimersomes as versatile cell membrane mimetics, and assays provide diagnostic tools for protein functionality. This work also provides guidelines for the design of cell separators, bioactive matrices, bioeffectors, and other biomedical applications.
糖基树状聚合物囊泡具有可编程的聚糖表面展示，与人工工程化的半乳糖凝集素一起，用于理解具有同源二聚体和串联重复类型展示的人类凝集素的生理意义。拓扑表面呈现模式和聚糖密度影响多价碳水化合物-蛋白质相互作用介导的囊泡聚集。通过两个碳水化合物结合位点的共价连接增强了同源二聚体凝集素的交联能力。这些发现突出了糖基树状聚合物囊泡作为多功能细胞膜模拟物的价值，并且该测定法为蛋白质功能提供了诊断工具。这项工作还为细胞分离器、生物活性基质、生物效应物和其他生物医学应用的设计提供了指导。
