Ichinose Kunihiro, Arima Kazuhiko, Ushigusa Takeshi, Nishino Ayako, Nakashima Yoshikazu, Suzuki Takahisa, Horai Yoshiro, Nakajima Hideki, Kawashiri Shin-Ya, Iwamoto Naoki, Tamai Mami, Nakamura Hideki, Origuchi Tomoki, Motomura Masakatsu, Kawakami Atsushi
Department of Immunology and Rheumatology, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Department of Public Health, Nagasaki University Graduate School of Biomedical Sciences, Japan.
Clin Immunol. 2015 Apr;157(2):114-20. doi: 10.1016/j.clim.2015.01.010. Epub 2015 Feb 3.
Neuropsychiatric systemic lupus erythematosus (NPSLE) is a serious complication in SLE. Although the mechanism of NPSLE remains unclear, cytokines and chemokines are considered to be involved in their pathogenesis. Here we used Bio-Plex Pro assays to examine 27 types of cytokines and chemokines in the cerebrospinal fluid (CSF) of 32 NPSLE patients. We used the CSF of 20 patients with multiple sclerosis (MS) and 22 patients with neuromyelitis optica (NMO) as a disease control group. Fourteen of 27 cytokines/chemokines were significantly higher in the NPSLE patients compared to the MS/NMO patients. We could identify six "minimum predictive markers" by using a weighted-voting algorithm that could distinguish NPSLE from MS and NMO: interleukin (IL)-17, IL-2, interferon (IFN)-γ, IL-5, basic fibroblast growth factor (FGF)-basic and IL-15. The determination of various types of CSF cytokine profiles may contribute to the diagnosis of NPSLE and may help elucidate the mechanisms underlying this disease.
神经精神性系统性红斑狼疮（NPSLE）是系统性红斑狼疮（SLE）的一种严重并发症。尽管NPSLE的发病机制尚不清楚，但细胞因子和趋化因子被认为参与了其发病过程。在此，我们使用Bio-Plex Pro检测法对32例NPSLE患者脑脊液（CSF）中的27种细胞因子和趋化因子进行了检测。我们将20例多发性硬化症（MS）患者和22例视神经脊髓炎（NMO）患者的脑脊液作为疾病对照组。与MS/NMO患者相比，27种细胞因子/趋化因子中的14种在NPSLE患者中显著升高。通过使用加权投票算法，我们可以识别出六种“最小预测标志物”，这些标志物能够将NPSLE与MS和NMO区分开来：白细胞介素（IL）-17、IL-2、干扰素（IFN）-γ、IL-5、碱性成纤维细胞生长因子（FGF）-碱性和IL-15。各种类型脑脊液细胞因子谱的测定可能有助于NPSLE的诊断，并可能有助于阐明该疾病的潜在机制。
