Carey D, Nuttall P A
Natural Environment Research Council, Institute of Virology, Oxford, U.K.
Acta Virol. 1989 Jan;33(1):15-23.
Antigenic relationships between the four subgroups of the Kemerovo serogroup of tick-borne orbiviruses were examined. Kemerovo (KEM subgroup), Broadhaven (BRD) and Wexford (WEX) [Great Island (GI) sub-group], Chenuda (CNU sub-group), and Wad Medani (WM sub-group) viruses cross-reacted in immunofluorescence tests. Complement fixation tests (CFT) revealed that KEM virus was more closely related to BRD and WEX viruses than to either CNU or WM viruses. By cross-neutralization, all the viruses were shown to be distinct; only BRD and WEX showed slight cross-reaction. In Vero cells infected with either KEM, BRD, WEX, or CNU viruses, 10 major (p1 to p10) and a variable number of minor virus-induced 35S-labelled polypeptides were detected. Comparison of the polypeptides precipitated by homologous AF revealed close similarities between BRD and WEX viruses but obvious differences between these two viruses and KEM and CNU viruses. In cross-reactions, p6 of KEM and CNU viruses, and p7 of BRD and WEX viruses were immunoprecipitated. Immunofluorescence and immunoprecipitation tests appeared to identify an inter-group specific antigen that was distinct from an intra-group specific antigen detected by CFT. The results support division of KEM-related viruses into 3 new serogroups - KEM (comprising KEM and GI subgroups), CNU and WM - and indicate that a 38 to 43kD polypeptide carries the major inter-group specific antigen.
研究了蜱传环状病毒克麦罗沃血清群四个亚组之间的抗原关系。克麦罗沃（KEM亚组）、布罗德黑文（BRD）和韦克斯福德（WEX）[大岛（GI）亚组]、切努达（CNU亚组）以及瓦德迈达尼（WM亚组）病毒在免疫荧光试验中发生交叉反应。补体结合试验（CFT）显示，KEM病毒与BRD和WEX病毒的关系比与CNU或WM病毒更为密切。通过交叉中和试验，所有病毒均表现出明显差异；只有BRD和WEX显示出轻微交叉反应。在感染KEM、BRD、WEX或CNU病毒的Vero细胞中，检测到10种主要（p1至p10）以及数量不等的次要病毒诱导的35S标记多肽。同源抗血清沉淀的多肽比较显示，BRD和WEX病毒之间有密切相似性，但这两种病毒与KEM和CNU病毒之间存在明显差异。在交叉反应中，KEM和CNU病毒的p6以及BRD和WEX病毒的p7被免疫沉淀。免疫荧光和免疫沉淀试验似乎鉴定出一种组间特异性抗原，它不同于CFT检测到的组内特异性抗原。结果支持将与KEM相关的病毒分为3个新血清群——KEM（包括KEM和GI亚组）
