Moss S R, Ayres C M, Nuttall P A
NERC Institute of Virology, Oxford, U.K.
J Gen Virol. 1988 Nov;69 ( Pt 11):2721-7. doi: 10.1099/0022-1317-69-11-2721.
The geographical distribution of members of the Great Island (GI) subgroup in the Kemerovo serogroup of orbiviruses extends from the Arctic to the Sub-antarctic. To examine the gene pool size of this group, five topotypes whose origins ranged from Iceland in the northern hemisphere to Macquarie Island in the Southern Ocean were tested for their ability to reassort in vitro. All the isolates were distinguishable by plaque reduction neutralization tests, and their genome profile in polyacrylamide gels. They showed high frequency reassortment following dual infection of cell cultures with temperature-sensitive (ts) mutants and/or wild-type virus. Analysis of the dsRNA profile of the reassortants by PAGE confirmed the observation from reassortment assays that the Great Island subgroup constitutes a single gene pool. A seventh reassortment group was identified, distinct from the six groups previously described. The ts lesions for reassortment groups I, V and VII were considered to be in genome segments 9, 3 and 2, respectively. Segment 6 of GI virus (in contrast to segment 5 of Broadhaven and Wexford viruses) was shown to be the major genetic determinant of serotype specificity.
环状病毒克麦罗沃血清群中大岛（GI）亚组成员的地理分布范围从北极延伸至亚南极。为了研究该亚组的基因库大小，对5种原型毒株进行了体外重配能力测试，这些毒株的来源范围从北半球的冰岛到南大洋的麦夸里岛。所有分离株通过蚀斑减少中和试验及其在聚丙烯酰胺凝胶中的基因组图谱均可区分。在用温度敏感（ts）突变体和/或野生型病毒双重感染细胞培养物后，它们显示出高频重配。通过PAGE对重配体的dsRNA图谱进行分析，证实了重配试验的观察结果，即大岛亚组构成一个单一的基因库。鉴定出了第七个重配组，与先前描述的六个组不同。重配组I、V和VII的ts损伤分别被认为位于基因组片段9、3和2中。GI病毒的片段6（与布罗德黑文病毒和韦克斯福德病毒的片段5相反）被证明是血清型特异性的主要遗传决定因素。
