Ding Yin-Huan, Guo Jian-Hong, Hu Qiong-Ying, Jiang Wei, Wang Kai-Zheng
Department of Laboratory Medicine, Affiliated Hospital of Luzhou Medical College, 25 Taiping Street, Luzhou, 646000, China.
The People's Hospital of Luzhou, 61 Zhongxiao Street, Luzhou, 646000, China.
Cell Biochem Biophys. 2015 Jul;72(3):799-805. doi: 10.1007/s12013-015-0536-5.
This study was devised to identify potential biomarkers of schizophrenia (SP) using proteomics techniques. We obtained 44 serum specimens from patients with SP, 26 specimens from patients with depression, and 40 specimens from healthy controls. Immobilized metal affinity capture protein chips (IMAC30) and surface-enhanced laser desorption-ionization time-of-flight mass spectrometry were used to isolate and obtain mass spectrometric data of differentially expressed serum proteins. The sequences of the peaks discrepant among the study groups were obtained using matrix-assisted laser desorption/ionization mass spectrometry and proteins identified using Mascot database. In the SP group, there were 91 protein peaks that were different from other study groups at the p value of <0.05 and 54 peaks different at the p value of <0.01. Two protein peaks at the mass-to-charge ratio of 1,207.41 and 1,466.78 were markedly different among the study groups, with the lowest expression in specimens from patients with SP. The amino acid sequences were, respectively, Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg (EGDFLAEGGGVR) and Asp-Ser-Gly-Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg (DSGEGDFLAEGGGVR). These proteins were identified as the N-terminal fragments of fibrinogen. In conclusion, these biomarker proteins may be useful for molecular diagnosis of SP.
本研究旨在利用蛋白质组学技术鉴定精神分裂症（SP）的潜在生物标志物。我们从SP患者中获取了44份血清样本，从抑郁症患者中获取了26份样本，以及从健康对照中获取了40份样本。使用固定化金属亲和捕获蛋白芯片（IMAC30）和表面增强激光解吸电离飞行时间质谱法来分离并获得差异表达血清蛋白的质谱数据。使用基质辅助激光解吸/电离质谱法获得研究组间差异峰的序列，并使用Mascot数据库鉴定蛋白质。在SP组中，有91个蛋白峰在p值<0.05时与其他研究组不同，54个峰在p值<0.01时不同。质荷比为1207.41和1466.78的两个蛋白峰在研究组间差异显著，在SP患者样本中的表达最低。氨基酸序列分别为Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg（EGDFLAEGGGVR）和Asp-Ser-Gly-Glu-Gly-Asp-Phe-Leu-Ala-Glu-Gly-Gly-Gly-Val-Arg（DSGEGDFLAEGGGVR）。这些蛋白质被鉴定为纤维蛋白原的N端片段。总之，这些生物标志物蛋白可能对SP的分子诊断有用。
