Ma Kai, Huang Zhifeng, Ma Jianfeng, Shao Longquan, Wang Huiming, Wang Yanliang
Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Medical College of Zhejiang University, Hangzhou 310006, Zhejiang Province, China ; Department of Oral and Maxillofacial Surgery, Affiliated Hospital of Stomatology, Wenzhou Medical College, Wenzhou 325027, Zhejiang Province, China.
School of Pharmacy, Wenzhou Medical College, Wenzhou 325000, Zhejiang Province, China.
Neural Regen Res. 2012 Jun 15;7(17):1293-8. doi: 10.3969/j.issn.1673-5374.2012.17.002.
The present study observed sciatic nerve and gastrocnemius muscle changes in denervated rats using morphology methods, and assessed expression of perlecan, an extracellular matrix component, which is located at the skeletal muscle cell surface as acetylcholine esterase, as well as synaptophysin, a synaptic marker. Results showed degeneration and inflammation following transection of the sciatic nerve. In addition, the sciatic nerve-dominated skeletal muscle degenerated with mild inflammation, indicating that skeletal muscle atrophy primarily contributed to denervation-induced nutritional disturbances. With prolonged injury time (1-4 weeks post-injury), perlecan expression gradually decreased and reached the lowest level at 4 weeks, but synaptophysin expression remained unchanged after denervation. Results suggested that perlecan expression was more sensitive to denervation and reflected regional extracellular matrix changes following denervation.
本研究采用形态学方法观察去神经大鼠坐骨神经和腓肠肌的变化，并评估位于骨骼肌细胞表面作为乙酰胆碱酯酶的细胞外基质成分核心蛋白聚糖以及突触标记物突触素的表达。结果显示坐骨神经横断后出现变性和炎症。此外，由坐骨神经支配的骨骼肌发生变性并伴有轻度炎症，表明骨骼肌萎缩主要是由去神经引起的营养紊乱所致。随着损伤时间延长（损伤后1 - 4周），核心蛋白聚糖表达逐渐降低，在4周时达到最低水平，但去神经后突触素表达保持不变。结果表明，核心蛋白聚糖表达对去神经更为敏感，反映了去神经后局部细胞外基质的变化。
