Department of Biology, Faculty of Sciences, University of Guilan, Rasht, Iran.
J Mol Neurosci. 2011 Oct;45(2):87-93. doi: 10.1007/s12031-010-9393-2. Epub 2010 Jun 1.
Fibroblast growth factor has been implicated in cerebral cortical cell proliferation, migration, survival and differentiation. In the nervous system, proteoglycans (PGs) modulate growth factor activities and cell proliferation. Perlecan is a large PG and is expressed during the prenatal stages of brain development. Perlecan can promote fibroblast growth factor-2 (FGF-2) activity. In this study the effects of FGF-2 on neurogenesis and perlecan expression in the cerebral cortex have been studied. FGF-2 and anti-FGF-2 antibody were administered to 1-day-old mouse pups through the cisterna magna. Two days after injection, the pups were harvested and brains were removed for further analysis. We used western blotting and immunohistochemistry in order to analyze perlecan expression and neurogenesis in the cerebral cortex. We showed that the thickness of cerebral cortex, the number of cells produced in the cerebral cortex and perlecan expression are increased in response to injection of FGF-2 and decreased in response to infusion of anti-FGF-2 antibody as compared to controls. In conclusion, the results from this study suggest that FGF-2 increases neural cell production and perlecan expression in the developing cerebral cortex. It is also concluded that FGF-2 is an important factor in the cerebral cortical development, stimulating neuronal precursor proliferation.
成纤维细胞生长因子已被牵涉到大脑皮质细胞的增殖、迁移、存活和分化中。在神经系统中，蛋白聚糖（PGs）调节生长因子的活性和细胞增殖。基底膜硫酸乙酰肝素蛋白聚糖是一种大型的 PG，在大脑发育的产前阶段表达。基底膜硫酸乙酰肝素蛋白聚糖可以促进成纤维细胞生长因子-2（FGF-2）的活性。在这项研究中，研究了 FGF-2 对大脑皮层神经发生和基底膜硫酸乙酰肝素蛋白聚糖表达的影响。通过脑池向 1 日龄的小鼠幼崽中给予 FGF-2 和抗 FGF-2 抗体。注射后两天，收获幼崽并取出大脑进行进一步分析。我们使用 Western blot 和免疫组织化学分析来分析大脑皮层中的基底膜硫酸乙酰肝素蛋白聚糖表达和神经发生。结果表明，与对照组相比，FGF-2 注射会增加大脑皮层的厚度、大脑皮层中产生的细胞数量和基底膜硫酸乙酰肝素蛋白聚糖的表达，而抗 FGF-2 抗体的输注则会减少这些变化。总之，这项研究的结果表明，FGF-2 增加了发育中的大脑皮层中的神经细胞产生和基底膜硫酸乙酰肝素蛋白聚糖的表达。还得出结论，FGF-2 是大脑皮层发育的重要因素，刺激神经元前体的增殖。
