Gao Qi, Liang Yonghui, Yang Xiaoyu, Liu Guifeng, Li Xiaoxue, Zhu Benqing, Liu Jian, Yang Maoguang, Xia Weiwei, Dong Jian, Jiao Jianhang
Team of Spine and Spinal Cord, Department of Orthopedics, China-Japan Friendship Hospital, Jilin University, Changchun 130033, Jilin Province, China.
Neural Regen Res. 2012 Jul 15;7(20):1534-9. doi: 10.3969/j.issn.1673-5374.2012.20.002.
New Zealand rabbits were randomly divided into an ischemia group (occlusion of the abdominal aorta for 60 minutes), an ischemia-reperfusion group (occlusion of the abdominal aorta for 60 minutes followed by 48 hours of reperfusion) and a sham-surgery group. Two-dimensional gel electrophoresis detected 49 differentially expressed proteins in spinal cord tissue from the ischemia and ischemia/ reperfusion groups and 23 of them were identified by mass spectrometry. In the ischemia group, the expression of eight proteins was up regulated, and that of the remaining four proteins was down regulated. In the ischemia/reperfusion group, the expression of four proteins was up regulated, and that of two proteins was down regulated. In the sham-surgery group, only one protein was detected. In the ischemia and ischemia/reperfusion groups, four proteins overlapped between groups with the same differential expression, including three that were up regulated and one down regulated. These proteins were related to energy metabolism, cell defense, inflammatory mechanism and cell signaling.
新西兰兔被随机分为缺血组（腹主动脉阻断60分钟）、缺血再灌注组（腹主动脉阻断60分钟后再灌注48小时）和假手术组。二维凝胶电泳检测到缺血组和缺血/再灌注组脊髓组织中有49种差异表达蛋白，其中23种通过质谱鉴定。在缺血组中，8种蛋白表达上调，其余4种蛋白表达下调。在缺血/再灌注组中，4种蛋白表达上调，2种蛋白表达下调。在假手术组中，仅检测到1种蛋白。在缺血组和缺血/再灌注组中，两组间有4种蛋白具有相同的差异表达，其中3种上调，1种下调。这些蛋白与能量代谢、细胞防御、炎症机制和细胞信号传导有关。
