Laboratory of Entomology, Wageningen University, P.O. Box 16, 6700 AA Wageningen, The Netherlands ; Laboratory of Plant Physiology, Wageningen University, P.O. Box 16, 6700 AA Wageningen, The Netherlands ; Plant Research International, Wageningen University and Research Center, P.O. Box 16, 6700 AA Wageningen, The Netherlands.
Laboratory of Entomology, Wageningen University, P.O. Box 16, 6700 AA Wageningen, The Netherlands.
Plant Methods. 2015 Jan 30;11:4. doi: 10.1186/s13007-015-0044-z. eCollection 2015.
Piercing-sucking insects are major vectors of plant viruses causing significant yield losses in crops. Functional genomics of plant resistance to these insects would greatly benefit from the availability of high-throughput, quantitative phenotyping methods.
We have developed an automated video tracking platform that quantifies aphid feeding behaviour on leaf discs to assess the level of plant resistance. Through the analysis of aphid movement, the start and duration of plant penetrations by aphids were estimated. As a case study, video tracking confirmed the near-complete resistance of lettuce cultivar 'Corbana' against Nasonovia ribisnigri (Mosely), biotype Nr:0, and revealed quantitative resistance in Arabidopsis accession Co-2 against Myzus persicae (Sulzer). The video tracking platform was benchmarked against Electrical Penetration Graph (EPG) recordings and aphid population development assays. The use of leaf discs instead of intact plants reduced the intensity of the resistance effect in video tracking, but sufficiently replicated experiments resulted in similar conclusions as EPG recordings and aphid population assays. One video tracking platform could screen 100 samples in parallel.
Automated video tracking can be used to screen large plant populations for resistance to aphids and other piercing-sucking insects.
刺吸式昆虫是导致作物产量严重损失的主要植物病毒载体。植物对这些昆虫的抗性的功能基因组学将极大地受益于高通量、定量表型分析方法的可用性。
我们开发了一种自动视频跟踪平台，可量化蚜虫在叶盘上的取食行为，以评估植物抗性水平。通过分析蚜虫的运动，估计了蚜虫对植物的穿透开始和持续时间。作为一个案例研究，视频跟踪证实了生菜品种“科巴纳”对 Nasonovia ribisnigri（Mosely），生物型 Nr：0 的近乎完全抗性，并揭示了拟南芥品系 Co-2 对桃蚜的定量抗性。视频跟踪平台与电穿透图（EPG）记录和蚜虫种群发展测定进行了基准测试。与使用完整植物相比，使用叶盘减少了视频跟踪中抗性效应的强度，但足够的重复实验得出了与 EPG 记录和蚜虫种群测定相似的结论。一个视频跟踪平台可以同时筛选 100 个样本。
自动视频跟踪可用于筛选对蚜虫和其他刺吸式昆虫具有抗性的大量植物群体。
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