Nakano Y, Tanaka E, Kato C, Kimura K, Horikoshi K
Laboratory of Biochemistry, College of Science, Rikkyo University, Tokyo, Japan.
FEMS Microbiol Lett. 1989 Jan 1;48(1):81-6. doi: 10.1016/0378-1097(89)90151-1.
The glutamine synthetase (GS) gene from Bacillus subtilis PCI 219 was cloned in Escherichia coli using the vector pBR329. A plasmid, pSGS2, was isolated from a glnA+ transformant and the cloned GS gene was found to be located in a 3.6 kb DNA fragment. The nucleotide sequence of a 1.8 kb segment encoding the GS was determined. This segment showed an open reading frame which would encode a polypeptide of 444 amino acids. The amino acid sequence of this GS gene product has higher homology with that of the Clostridium acetobutylicum GS than that of the E. coli GS.
利用载体pBR329，将枯草芽孢杆菌PCI 219的谷氨酰胺合成酶（GS）基因克隆到大肠杆菌中。从谷氨酰胺合成酶基因（glnA）阳性转化体中分离出一个质粒pSGS2，发现克隆的GS基因位于一个3.6 kb的DNA片段中。测定了编码GS的1.8 kb片段的核苷酸序列。该片段显示出一个开放阅读框，可编码一个由444个氨基酸组成的多肽。该GS基因产物的氨基酸序列与丙酮丁醇梭菌GS的氨基酸序列的同源性高于与大肠杆菌GS的同源性。
