Wray L V, Fisher S H
Department of Medical Microbiology and Immunology, University of Kentucky, Lexington 40436.
Gene. 1988 Nov 30;71(2):247-56. doi: 10.1016/0378-1119(88)90041-8.
The Streptomyces coelicolor glutamine synthetase (GS) structural gene (glnA) was cloned by complementing the glutamine growth requirement of an Escherichia coli strain containing a deletion of its glnALG operon. Expression of the cloned S. coelicolor glnA gene in E. coli cells was found to require an E. coli plasmid promoter. The nucleotide sequence of an S. coelicolor 2280-bp DNA segment containing the glnA gene was determined and the complete glnA amino acid sequence deduced. Comparison of the derived S. coelicolor GS protein sequence with the amino acid sequences of GS from other bacteria suggests that the S. coelicolor GS protein is more similar to the GS proteins from Gram-negative bacteria than it is with the GS proteins from two Gram-positive bacteria, Bacillus subtilis and Clostridium acetobutylicum.
通过补充一株缺失谷氨酰胺合成酶操纵子（glnALG）的大肠杆菌菌株对谷氨酰胺的生长需求，克隆了天蓝色链霉菌谷氨酰胺合成酶（GS）结构基因（glnA）。发现克隆的天蓝色链霉菌glnA基因在大肠杆菌细胞中的表达需要一个大肠杆菌质粒启动子。测定了包含glnA基因的一段2280bp天蓝色链霉菌DNA片段的核苷酸序列，并推导了完整的glnA氨基酸序列。将推导的天蓝色链霉菌GS蛋白序列与其他细菌的GS氨基酸序列进行比较，结果表明，天蓝色链霉菌GS蛋白与革兰氏阴性菌的GS蛋白比与两种革兰氏阳性菌枯草芽孢杆菌和丙酮丁醇梭菌的GS蛋白更为相似。
