Perez-Toralla Karla, Pekin Deniz, Bartolo Jean-François, Garlan Fanny, Nizard Philippe, Laurent-Puig Pierre, Baret Jean-Christophe, Taly Valérie
Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France.
Université Paris Sorbonne Cité, Inserm UMR-S1147, 45, rue des Saints-Pères, 75270 Paris, France - Droplets membranes and interfaces, Max Planck institute for dynamics and self-organization, Am Fassberg 17, D-37077 Göttingen, Allemagne.
Med Sci (Paris). 2015 Jan;31(1):84-92. doi: 10.1051/medsci/20153101017. Epub 2015 Feb 6.
Polymerase chain reaction based techniques have been widely used in laboratory settings. Several applications in oncology, virology or prenatal diagnosis require highly sensitive detection methods, which cannot be achieved with conventional techniques. Digital PCR (dPCR) was developed from the association of PCR and limiting dilution procedures. It is based on the compartmentalization of DNA molecules in small volumes. Controlling the size and the content of each compartment is crucial to obtain a high sensitivity with a single molecule resolution. Microfluidics offers promising tools to isolate DNA fragments such as microdroplets, microchambers or microwells with volumes ranging from few picoliters to nanoliters. The review provides an overview of recent developments of microfluidics dPCR platforms and how this technology can influence the management of cancer patients.
基于聚合酶链反应的技术已在实验室环境中广泛使用。肿瘤学、病毒学或产前诊断中的一些应用需要高度灵敏的检测方法，而传统技术无法实现这一点。数字PCR（dPCR）是由PCR与有限稀释程序结合发展而来的。它基于将DNA分子分隔在小体积中。控制每个隔室的大小和内容对于以单分子分辨率获得高灵敏度至关重要。微流控技术提供了很有前景的工具来分离DNA片段，如体积从几皮升至纳升不等的微滴、微腔或微孔。本文综述概述了微流控dPCR平台的最新进展以及该技术如何影响癌症患者的管理。
