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采用高效液相色谱-质谱联用技术鉴定旱金莲(Tropaeolum majus)花瓣中的酚类化合物并测定其氧自由基吸收能力(ORAC)

Identification of phenolic compounds in petals of nasturtium flowers (Tropaeolum majus) by high-performance liquid chromatography coupled to mass spectrometry and determination of oxygen radical absorbance capacity (ORAC).

作者信息

Garzón G Astrid, Manns David C, Riedl Ken, Schwartz Steven J, Padilla-Zakour Olga

机构信息

Departamento de Quı́mica, Universidad Nacional de Colombia , AA 14490 Bogotá, Colombia.

出版信息

J Agric Food Chem. 2015 Feb 18;63(6):1803-11. doi: 10.1021/jf503366c. Epub 2015 Feb 9.

DOI:10.1021/jf503366c
PMID:25659835
Abstract

The contents and profile of polyphenols were analyzed in edible petals of nasturtium flowers (Tropaeolum majus) of three colors, and their oxygen radical absorbance capacities (ORAC) were compared. Three primary anthocyanins (ACNs) and 15 non-ACN phenolic compounds including hydroxycinammic acids (HCAs) and flavonoids (myricetin, quercetin, and kaempferol derivatives) were detected. Anthocyanin concentration was within 31.9 ± 21.7 and 114.5 ± 2.3 mg cyanidin-3-glucoside (cy-3-glu)/100 g fresh weight (FW) in yellow and red petals, respectively. The concentration of HCAs varied between 33.3 ± 7.1 and 235.6 ± 8.1 mg chlorogenic acid equivalents/100 g FW for red and yellow flowers, respectively. Red flowers had the highest level of flavonoids (315.1 ± 2.4 mg myricetin equivalents/100 g FW) and the highest ORAC radical-scavenging activity. These results show the diversity and abundance of polyphenolic compounds in nasturtium flowers, which could be the basis for applications in functional foods, cosmetics, and pharmaceuticals.

摘要

对三种颜色的旱金莲(Tropaeolum majus)可食用花瓣中的多酚含量和成分进行了分析,并比较了它们的氧自由基吸收能力(ORAC)。检测到三种主要花青素(ACN)和15种非ACN酚类化合物,包括羟基肉桂酸(HCA)和黄酮类化合物(杨梅素、槲皮素和山奈酚衍生物)。黄色和红色花瓣中的花青素浓度分别在31.9±21.7和114.5±2.3毫克矢车菊素-3-葡萄糖苷(cy-3-glu)/100克鲜重(FW)范围内。红色和黄色花朵中HCA的浓度分别在33.3±7.1和235.6±8.1毫克绿原酸当量/100克FW之间变化。红色花朵的黄酮类化合物含量最高(315.1±2.4毫克杨梅素当量/100克FW),且具有最高的ORAC自由基清除活性。这些结果表明旱金莲花瓣中多酚化合物的多样性和丰富性,这可能是其在功能性食品、化妆品和药品中应用的基础。

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