Phunchago Nattaporn, Wattanathorn Jintanaporn, Chaisiwamongkol Kowit, Muchimapura Supaporn, Thukham-Mee Wipawee
Department of Physiology (Neuroscience Program), Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen, Thailand.
Integrative Complementary Alternative Medicine Research and Development Center, Khon Kaen University, Khon Kaen, Thailand; Department of Physiology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand.
J Acupunct Meridian Stud. 2015 Feb;8(1):23-9. doi: 10.1016/j.jams.2014.11.008. Epub 2014 Dec 6.
Currently, the therapeutic strategy against memory deficit induced by alcoholism is not satisfactory and is expensive. Therefore, an effective, low-cost strategy is required. On the basis of the memory-enhancing effect of stimulation of the HT7 acupoint, we aimed to determine whether acupuncture at the HT7 acupoint can reduce alcoholism-induced memory impairment. The possible underlying mechanism was also explored. Alcoholism was induced in male Wistar rats weighing 180-220 g. The alcoholic rats received either acupuncture at HT7 or sham acupuncture for 1 minute bilaterally once daily for 14 days. Their spatial memory was assessed after 1 day, 7 days, and 14 days of treatment. At the end of the study, the malondialdehyde level and the activities of catalase, superoxide dismutase, glutathione peroxidase, and acetylcholinesterase enzymes in the hippocampus were determined using colorimetric assays. The results showed that acupuncture at HT7 significantly decreased the acetylcholinesterase activity and the malondialdehyde level, but increased the activities of catalase, superoxide dismutase, and glutathione peroxidase in the hippocampus. These results suggest that acupuncture at HT7 can effectively reduce the alcoholism-induced memory deficit. However, further studies concerning the detailed relationships between the location of the HT7 acupoint and the changes in the observed parameters are required.
目前，针对酒精中毒所致记忆缺陷的治疗策略并不令人满意且费用高昂。因此，需要一种有效且低成本的策略。基于刺激HT7穴位的记忆增强作用，我们旨在确定针刺HT7穴位是否能减轻酒精中毒所致的记忆损害。同时还探讨了可能的潜在机制。对体重180 - 220克的雄性Wistar大鼠诱导酒精中毒。酒精中毒大鼠每天接受双侧HT7针刺或假针刺1分钟，持续14天。在治疗1天、7天和14天后评估它们的空间记忆。在研究结束时，使用比色法测定海马中的丙二醛水平以及过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶和乙酰胆碱酯酶的活性。结果表明，针刺HT7可显著降低海马中的乙酰胆碱酯酶活性和丙二醛水平，但可提高过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶的活性。这些结果表明，针刺HT7可有效减轻酒精中毒所致的记忆缺陷。然而，需要进一步研究HT7穴位位置与观察到的参数变化之间的详细关系。
