Jittiwat Jinatta
Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Integrative Complimentary Alternative Medicine Research and Development Group, Khon Kaen University, Khon Kaen, Thailand.
J Acupunct Meridian Stud. 2017 Oct;10(5):324-330. doi: 10.1016/j.jams.2017.08.003. Epub 2017 Sep 6.
The burden of stroke is high and is continually increasing due to a dramatic growth in the world's elderly population. Novel therapeutic strategies are therefore required. The present study sought to determine the effect of laser acupuncture at GV20 on brain damage, oxidative-status markers in the cerebral cortex, and superoxide dismutase in the mitochondria of an animal model of focal ischemic stroke. Wistar rats, weighing 300-350 g, were divided into the following four groups: (1) control; (2) permanent occlusion of the right middle cerebral artery (Rt.MCAO) alone; (3) Rt.MCAO plus sham laser acupuncture; and (4) Rt.MCAO plus laser-acupuncture groups. Sham laser acupuncture or laser acupuncture was performed once daily at the GV20 (Baihui) acupoint for 14 days following Rt.MCAO. Half of the rats in each group were examined by 2,3,5-triphenyltetrazolium chloride staining to determine the brain infarct volume, while the other half were examined by biochemical assays to determine the malondialdehyde level, and the glutathione peroxidase, catalase, and superoxide-dismutase activities in the brain-cortex mitochondria. The results showed that laser acupuncture at GV20 significantly decreased the brain infarct volume and malondialdehyde level, and increased the catalase, glutathione peroxidase, and superoxide-dismutase activities in cerebral ischemic rats. In conclusion, laser acupuncture at GV20 decreases the brain infarct volume in cerebral ischemic rats, at least in part due to decreased oxidative stress. Further study is warranted to investigate other possible underlying mechanisms.
由于全球老年人口的急剧增长，中风的负担很重且持续增加。因此需要新的治疗策略。本研究旨在确定针刺督脉20穴（百会穴）对局灶性缺血性中风动物模型的脑损伤、大脑皮质氧化状态标志物以及线粒体中超氧化物歧化酶的影响。将体重300 - 350克的Wistar大鼠分为以下四组：（1）对照组；（2）单纯右侧大脑中动脉永久性闭塞组（Rt.MCAO）；（3）Rt.MCAO加假激光针刺组；（4）Rt.MCAO加激光针刺组。在Rt.MCAO后，每天在督脉20穴（百会穴）进行一次假激光针刺或激光针刺，持续14天。每组一半的大鼠通过2,3,5 - 氯化三苯基四氮唑染色检查以确定脑梗死体积，另一半通过生化分析检查以确定丙二醛水平以及大脑皮质线粒体中的谷胱甘肽过氧化物酶、过氧化氢酶和超氧化物歧化酶活性。结果表明，针刺督脉20穴可显著降低脑缺血大鼠的脑梗死体积和丙二醛水平，并提高过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶的活性。总之，针刺督脉20穴可减少脑缺血大鼠的脑梗死体积，至少部分原因是氧化应激降低。有必要进一步研究以探讨其他可能的潜在机制。
