State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
State Key Laboratory of Food Science and Technology, Synergetic Innovation Center of Food Safety and Nutrition, School of Food Science and Technology, Jiangnan University, 1800 Lihu Avenue, Wuxi 214122, People's Republic of China.
Bioresour Technol. 2015 Apr;181:275-82. doi: 10.1016/j.biortech.2015.01.009. Epub 2015 Jan 9.
Arachidonic acid (ARA) yield and productivity of Mortierella alpina mutant D20 were enhanced by integrating a fed-batch culture combined with a two-stage pH control strategy. Following a kinetic analysis of the whole fermentation process, a two-stage pH control strategy was developed in which the pH was maintained at 5.5 for the first 48 h and then shifted to 6.5 till the end of fermentation. Using this strategy, a maximum ARA production of 8.12 g/L was achieved. On the basis of pH control, the effects of fed-batch cultures on ARA productivity were further investigated. A maximum ARA productivity of 1.40 g/L/d was obtained with a two-stage constant-speed glucose feeding strategy, starting with a glucose concentration of 50 g/L. This strategy was simple and economical to operate, and it may be possible to apply this approach for large-scale industrial production of ARA.
通过整合分批培养和两段式 pH 控制策略，提高了高山被孢霉 D20 突变株的花生四烯酸（ARA）产量和生产效率。在对整个发酵过程进行动力学分析后，开发了一种两段式 pH 控制策略，即在发酵的前 48 h 将 pH 维持在 5.5，然后在发酵结束前将 pH 调至 6.5。采用该策略，ARA 的最高产量达到了 8.12 g/L。在此基础上，基于 pH 控制，进一步考察了分批培养对 ARA 生产效率的影响。采用两段式恒速葡萄糖补料策略，起始葡萄糖浓度为 50 g/L，可获得最高的 ARA 生产效率 1.40 g/L/d。该策略操作简单、经济，有望应用于大规模工业化生产 ARA。
