Oliveira Rafaela B, da Silveira Juliane Machado, Feltes Maria Manuela C, Robl Diogo, Ienczak Jaciane L
Department of Chemical Engineering and Food Engineering, Federal University of Santa Catarina, Florianopolis, Brazil.
Department of Food Science and Technology, Federal University of Santa Catarina, Florianopolis, Brazil.
Appl Biochem Biotechnol. 2025 Sep 12. doi: 10.1007/s12010-025-05369-z.
Polyunsaturated fatty acids (PUFAs) are essential dietary lipids, vital for health and cellular integrity, with growing applications in biofuel, food, and pharmaceuticals. In this sense, this work investigated the production of single cell oil (SCO) from newly isolated Mortierellaceae strains from Antarctica. These strains were identified through ITS region sequencing and one of them (Linnemannia sp. ACF038) was selected for scaling up studies. The study of the physiological characterization of the selected strain was made by assessing various parameters including pH, temperature, nitrogen sources, and concentrations through solid and flask cultures. Based on these results, it was possible to scale up and optimize the culture conditions for SCO production using bench-scale bioreactors with submerged cultures. This process involved the use of two different culture media and an in-depth kinetic study to identify the most effective strategy for SCO production. The study also examined the volumetric oxygen transfer coefficient and oxygen consumption rate. The results showed that the culture medium with high initial glucose content and sodium nitrate significantly increased lipid production, reaching 65% of lipid content. Linoleic acid (LA) comprised 42% of the lipids, indicating strong potential for large-scale LA production. Linnemannia sp. ACF038 proved to be a promising candidate for sustainable industrial LA production, meeting the rising demand for PUFAs.
多不饱和脂肪酸（PUFAs）是必需的膳食脂质，对健康和细胞完整性至关重要，在生物燃料、食品和制药领域的应用日益广泛。从这个意义上讲，这项工作研究了从新分离的南极被孢霉科菌株生产单细胞油（SCO）。通过ITS区域测序对这些菌株进行了鉴定，并选择其中一株（Linnemannia sp. ACF038）进行放大研究。通过固体和摇瓶培养评估包括pH、温度、氮源和浓度等各种参数，对所选菌株进行生理特性研究。基于这些结果，使用带有深层培养的实验室规模生物反应器，可以放大并优化SCO生产的培养条件。这个过程涉及使用两种不同的培养基以及深入的动力学研究，以确定SCO生产的最有效策略。该研究还考察了体积氧传递系数和耗氧率。结果表明，初始葡萄糖含量高且含有硝酸钠的培养基显著提高了脂质产量，脂质含量达到65%。亚油酸（LA）占脂质的42%，表明大规模生产LA具有很大潜力。Linnemannia sp. ACF038被证明是可持续工业生产LA的有前途的候选菌株，可满足对PUFAs不断增长的需求。
