Zhang D, Sun W, Feng G, Zhang F, Anbuchezhian R, Li Z, Jiang Q
Marine Biotechnology Laboratory, State Key Laboratory of Microbial Metabolism and School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, China.
Lett Appl Microbiol. 2015 May;60(5):504-12. doi: 10.1111/lam.12400. Epub 2015 Mar 12.
Marine sponges harbour dense and diverse micro-organisms which includes sulphate-reducing bacteria (SRB). SRB are known to play a key role in the cycling of marine elements. However, in contrast to carbon and nitrogen cycling bacteria, SRB associated with marine sponges are largely unexplored. In this study, we explored the phylogenetic diversity of the SRB associated with three shallow-water sponges Arenosclera heroni, Dysidea arenaria and Astrosclera willeyana from the South China Sea by cloning-and-sequencing approach of SRB 16S rRNA gene with specific primers. The results showed that SRB associated with sponges mainly belonged to the genus Desulfovibrio in the class Deltaproteobacteria, i.e. a total of 14 Desulfovibrio-related OTUs were obtained from three sponges. The exception is identical OTUs from different sponges. Each sponge species harboured a unique set of Desulfovibrio OTUs, with only a few shared OTUs observed between species, suggesting different species of Desulfovibrio in different species of sponges. Meanwhile, some Desulfovibrio OTUs had a low similarity (<97%) with related sequences in GenBank and phylogenetic analysis indicating novel Desulfovibrio symbionts in sponges. The results contribute to the overall understanding of the phylogenetic diversity of SRB associated with sponges.
To date, in contrast to carbon and nitrogen cycling bacteria, sulphate-reducing bacteria (SRB) associated with marine sponges are largely unexplored; little is known about the phylogenetic diversity of SRB in different species of sponges. In the present study, phylogenetically diverse sulphate-reducing Desulfovibrio communities, including potential sponge species-specific and novel SRB, were revealed to be associated with South China Sea demosponges by cloning-and-sequencing approach of SRB 16S rRNA gene.
海洋海绵体内栖息着密集且多样的微生物，其中包括硫酸盐还原菌（SRB）。已知SRB在海洋元素循环中起关键作用。然而，与参与碳和氮循环的细菌不同，与海洋海绵相关的SRB在很大程度上尚未得到研究。在本研究中，我们通过使用特异引物对SRB 16S rRNA基因进行克隆和测序的方法，探究了来自中国南海的三种浅水海绵——赫氏砂海绵（Arenosclera heroni）、砂质皮海绵（Dysidea arenaria）和威利氏星骨海绵（Astrosclera willeyana）——所关联的SRB的系统发育多样性。结果表明，与海绵相关的SRB主要属于δ-变形菌纲中的脱硫弧菌属，即从三种海绵中总共获得了14个与脱硫弧菌相关的操作分类单元（OTU）。不同海绵中存在相同OTU的情况除外。每种海绵物种都拥有一组独特的脱硫弧菌OTU，不同物种之间仅观察到少数共享的OTU，这表明不同海绵物种中的脱硫弧菌种类不同。同时，一些脱硫弧菌OTU与GenBank中的相关序列相似度较低（<97%），系统发育分析表明海绵中存在新的脱硫弧菌共生体。这些结果有助于全面了解与海绵相关的SRB的系统发育多样性。
迄今为止，与参与碳和氮循环的细菌不同，与海洋海绵相关的硫酸盐还原菌（SRB）在很大程度上尚未得到研究；对于不同海绵物种中SRB的系统发育多样性知之甚少。在本研究中，通过对SRB 16S rRNA基因进行克隆和测序的方法，揭示了与中国南海海绵纲海绵相关的系统发育多样的硫酸盐还原脱硫弧菌群落，包括潜在的海绵物种特异性和新的SRB。
