Siddiqui Nasir A, Alam Perwez, Al-Rehaily Adnan J, Al-Oqail Mai M, Parvez Mohammad Khalid
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia
Department of Pharmacognosy, College of Pharmacy, King Saud University, Riyadh, Kingdom of Saudi Arabia.
J Chromatogr Sci. 2015 May-Jun;53(5):824-9. doi: 10.1093/chromsci/bmu231. Epub 2015 Feb 5.
A simple, sensitive high-performance thin-layer chromatography (HPTLC) method was developed for the simultaneous quantification of biomarker bergenin and menisdaurin in the methanol extracts of aerial parts of Flueggea virosa (FVME). Chromatography was performed on glass-backed silica gel 60F254 HPTLC plates using dichloromethane: methanol as mobile phase. Scanning and quantification was done at UV absorption maxima of 260 nm. The system was found to give compact spot for bergenin and menisdaurin at Rf = 0.29 ± 0.01 and 0.16 ± 0.01, respectively. The linearity ranges for bergenin and menisdaurin were found to be the same (100-800 ng/spot) with correlation coefficients (R(2) values) of 0.997 and 0.999, respectively. The limit of detection for bergenin and menisdaurin was found to be 27 and 36.2 ng/band, respectively, while the limit of quantification was found to be 81 and 108 ng/band, respectively. Intra- and interday precisions (n = 6) for bergenin and menisdaurin were found to be 1.41-1.71 and 1.65-1.87%, and 1.68-1.89 and 1.75-1.93%, respectively. The percent recoveries were found to be 98.7-99.4 and 99.5-99.9%, respectively, for bergenin and menisdaurin. The percentage of bergenin and menisdaurin was found to be 15.25 and 4.22% (w/w), respectively, in FVME. The developed method permitted the simultaneous quantification of bergenin and menisdaurin and showed good resolution and separation from other constituents of extract; hence, the method can be used to standardize herbal formulations as well as bulk drugs for bergenin and menisdaurin.
建立了一种简单、灵敏的高效薄层色谱（HPTLC）法，用于同时定量分析了飞扬草地上部分甲醇提取物（FVME）中的生物标志物岩白菜素和山柑子瑞香素。在玻璃背衬硅胶60F254 HPTLC板上进行色谱分析，以二氯甲烷：甲醇为流动相。在260 nm的紫外吸收最大值处进行扫描和定量。该系统被发现分别在Rf = 0.29±0.01和0.16±0.01处为岩白菜素和山柑子瑞香素给出紧密斑点。岩白菜素和山柑子瑞香素的线性范围相同（100 - 800 ng/斑点），相关系数（R²值）分别为0.997和0.999。岩白菜素和山柑子瑞香素的检测限分别为27和36.2 ng/条带，而定量限分别为81和108 ng/条带。岩白菜素和山柑子瑞香素的日内和日间精密度（n = 6）分别为1.41 - 1.71%和1.65 - 1.87%，以及1.68 - 1.89%和1.75 - 1.93%。岩白菜素和山柑子瑞香素的回收率分别为98.7 - 99.4%和99.5 - 99.9%。在FVME中，岩白菜素和山柑子瑞香素的含量分别为15.25%和4.22%（w/w）。所建立的方法允许同时定量岩白菜素和山柑子瑞香素，并显示出良好的分辨率以及与提取物的其他成分的分离；因此，该方法可用于标准化含有岩白菜素和山柑子瑞香素的草药制剂以及原料药。
