Wieraszko A, Goldsmith G, Seyfried T N
Department of Biology, Boston College, Chestnut Hill, MA 02167.
Brain Res. 1989 Apr 24;485(2):244-50. doi: 10.1016/0006-8993(89)90567-2.
Schaffer collaterals of rat and mouse hippocampal slices were stimulated with bursts of pulses (300 Hz for 50 ms, 2-s intervals) for 30-s which caused a stable increase in the size of the population spike known as long-term potentiation. The release of adenosine triphosphate (ATP) was measured with a luciferase-luciferine system and the light emitted was recorded with a photomultiplier placed beneath a modified slice chamber. ATP release was observed shortly after the start of stimulation and was quantified by comparison with the response of standard solutions of ATP. No ATP release was observed in a Ca2+ free solution or after low frequency stimulation (1 Hz). Glutamate (2 mM), applied without electrical stimulation, did not evoke ATP release. Also, the glutamate receptor blocker, kynurenic acid (10 mM), did not block ATP release. It is concluded that ATP is released from electrically stimulated hippocampal slices from presynaptic nerve terminals in a calcium-dependent fashion and may play a role in the modulation of synaptic efficiency.
用脉冲串（300赫兹，持续50毫秒，间隔2秒）刺激大鼠和小鼠海马切片的沙费尔侧支30秒，这会导致群体峰电位大小稳定增加，即长时程增强。用荧光素酶 - 荧光素系统测量三磷酸腺苷（ATP）的释放，并使用置于改良切片室下方的光电倍增管记录发出的光。在刺激开始后不久就观察到了ATP释放，并通过与ATP标准溶液的响应进行比较来定量。在无钙溶液中或低频刺激（1赫兹）后未观察到ATP释放。在没有电刺激的情况下施加谷氨酸（2毫摩尔）不会引起ATP释放。此外，谷氨酸受体阻断剂犬尿氨酸（10毫摩尔）也不会阻断ATP释放。得出的结论是，ATP以钙依赖的方式从电刺激的海马切片的突触前神经末梢释放，并且可能在突触效率的调节中起作用。
