Hamann M, Attwell D
Department of Physiology, University College London, Gower Street, London WC1E 6BT, UK.
Eur J Neurosci. 1996 Jul;8(7):1510-5. doi: 10.1111/j.1460-9568.1996.tb01613.x.
ATP is thought to be a fast neurotransmitter in the medial habenula region of the brain, and may be coreleased with other transmitters, for example with glutamate in the hippocampus. We monitored ATP release in rat brain slices using the bioluminescent indicator system luciferin-luciferase. Electrical stimulation of the hippocampus, cerebellum or habenula led to ATP release, but this release was calcium-independent and was not blocked by tetrodotoxin, or by other agents found to block ATP release from red blood cells. Although calcium-dependent ATP release may occur in response to electrical stimulation, it appears to be overwhelmed by calcium-independent release, which may result from electroporation of cells close to the stimulating electrode. Consistent with this, uptake into cells of the fluorescent dye Lucifer yellow was promoted by electrical stimulation. Our data undermine a previous suggestion, based on use of the luciferin-luciferase technique, that ATP is synaptically released with glutamate in the hippocampus.
三磷酸腺苷（ATP）被认为是大脑内侧缰核区域的一种快速神经递质，并且可能与其他递质共同释放，例如在海马体中与谷氨酸共同释放。我们使用生物发光指示剂系统荧光素 - 荧光素酶监测大鼠脑片中ATP的释放。对海马体、小脑或缰核进行电刺激会导致ATP释放，但这种释放不依赖于钙，并且不受河豚毒素或其他已知可阻断红细胞中ATP释放的试剂的阻断。尽管电刺激可能会引发依赖钙的ATP释放，但它似乎被不依赖钙的释放所掩盖，后者可能是由靠近刺激电极的细胞电穿孔导致的。与此一致的是，电刺激促进了荧光染料路西法黄进入细胞。我们的数据推翻了之前基于荧光素 - 荧光素酶技术得出的一项推测，即海马体中ATP与谷氨酸通过突触释放。
