Choi Siyoung, Murphy William L
Materials Science Program, University of Wisconsin-Madison, Madison, WI USA.
Materials Science Program, University of Wisconsin-Madison, Madison, WI USA ; Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI USA. ; Tel: +1 608 262 2224 ; Department of Orthopedics and Rehabilitation, University of Wisconsin-Madison, Madison, WI USA ; AO Foundation Collaborative Research Center, Davos, Switzerland.
J Mater Chem. 2012 Dec 28;22(48):25288-25295. doi: 10.1039/C2JM33354F.
Previous studies have demonstrated the influence of calcium phosphate (CaP) mineral coating characteristics on cell attachment, proliferation, and differentiation. However, the wide range of mineral properties that can potentially influence cell behavior calls for an efficient platform to screen for the effects of specific mineral properties. To address this need, we have developed an efficient well-plate format to probe for the effects of mineral coating properties on stem cell behavior. Specifically, here we systematically controlled mineral coating morphology by modulating ion concentrations in modified simulated body fluids (mSBF) during mineral nucleation and growth. We found that mineral micro-morphology could be gradually changed from spherulitic, to plate-like, to net-like depending on [Ca] and [PO] in mSBF solutions, while other mineral properties (Ca/P ratio, crystallinity, dissolution rate) remained constant. Differences in mineral morphology resulted in significant differences in stem cell attachment and expansion . These findings suggest that an enhanced throughput mineral coating format may be useful to identify mineral coating properties for optimal stem cell attachment and expansion, which may ultimately permit efficient intraoperative seeding of patient derived stem cells.
先前的研究已经证明了磷酸钙（CaP）矿物涂层特性对细胞附着、增殖和分化的影响。然而，广泛的可能影响细胞行为的矿物特性需要一个有效的平台来筛选特定矿物特性的影响。为了满足这一需求，我们开发了一种高效的微孔板形式来探究矿物涂层特性对干细胞行为的影响。具体而言，在这里我们通过在矿物成核和生长过程中调节改良模拟体液（mSBF）中的离子浓度来系统地控制矿物涂层形态。我们发现，根据mSBF溶液中的[Ca]和[PO]，矿物微观形态可以从球状逐渐变为板状，再变为网状，而其他矿物特性（Ca/P比、结晶度、溶解速率）保持不变。矿物形态的差异导致干细胞附着和扩增的显著差异。这些发现表明，一种通量更高的矿物涂层形式可能有助于识别出有利于最佳干细胞附着和扩增的矿物涂层特性，这最终可能允许对患者来源的干细胞进行高效的术中接种。
