聚左旋乳酸/无定形磷酸钙生物可吸收支架在冠状动脉中引起的炎症比聚左旋乳酸支架少。

Poly-l-lactic acid/amorphous calcium phosphate bioabsorbable stent causes less inflammation than poly-l-lactic acid stent in coronary arteries.

作者信息

Kong Lingting, Liu Wenbo, Yan Ge, Li Qiang, Yang Haiyan, Yu Fengxia, Song Haoming

机构信息

Department of Emergency, Yantaishan Hospital Yantai 264000, China.

Department of Cardiology, Yantaishan Hospital Yantai 264000, China.

出版信息

Int J Clin Exp Med. 2014 Dec 15;7(12):5317-23. eCollection 2014.

PMID:25664037

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4307484/

Abstract

AIM

Poly-l-lactic acid (PLLA) based bioabsorbable stents with or without amorphous calcium phosphate (ACP) were implanted and compared the inflammation in coronary arteries.

METHODS

6 PLLA and 6 PLLA/ACP based paclitaxel-eluting stents were randomly implanted into the coronary arteries of 12 healthy mini-pigs. The segments with stent were used to evaluate inflammation score and endothelialization score by hematoxylin-eosin staining.

RESULTS

At the 28th day after stent implantation, no in-stent restenosis or stent thrombosis was found in both PLLA and PLLA/ACP group. Histological analysis indicated that the inflammation score in PLLA/ACP group was less than that of in PLLA group (2.20±0.42 vs. 2.80±0.48, P<0.05). Consist with that, the expression of NF-κB was lower in PLLA/ACP group. The results from immunohistochemistry showed that the expressions of endothelial nitric oxide synthase (eNOS) and CD 31 in PLLA/ACP group were dramatically higher than those in PLLA group respectively. The serum levels of vascular endothelial growth factor (VEGF) and nitric oxide (NO) in PLLA/ACP group were significantly higher than those in PLLA group respectively (509.86±49.37 pg/ml vs. 322.04±35.16 pg/ml and 139.46±7.52 μmol/L vs. 29.55±16.55 μmol/L, P<0.05).

CONCLUSION

The application of ACP helps to reduce the inflammation caused by PLLA, and is also helpful in endothelial formation and function for PLLA-based bioabsorbable stent.

摘要

目的

植入含或不含无定形磷酸钙（ACP）的聚左旋乳酸（PLLA）基生物可吸收支架，并比较冠状动脉中的炎症情况。

方法

将6个基于PLLA的和6个基于PLLA/ACP的紫杉醇洗脱支架随机植入12只健康小型猪的冠状动脉中。使用苏木精-伊红染色法对有支架的节段进行炎症评分和内皮化评分评估。

结果

在支架植入后第28天，PLLA组和PLLA/ACP组均未发现支架内再狭窄或支架血栓形成。组织学分析表明，PLLA/ACP组的炎症评分低于PLLA组（2.20±0.42对2.80±0.48，P<0.05）。与此一致，PLLA/ACP组中核因子κB（NF-κB）的表达较低。免疫组织化学结果显示，PLLA/ACP组中内皮型一氧化氮合酶（eNOS）和CD 31的表达分别显著高于PLLA组。PLLA/ACP组中血管内皮生长因子（VEGF）和一氧化氮（NO）的血清水平分别显著高于PLLA组（509.86±49.37 pg/ml对322.04±35.16 pg/ml以及139.46±7.52 μmol/L对29.55±16.55 μmol/L，P<0.05）。