Bioengineering College of Chongqing University and 111 Project Laboratory of Biomechanics, Tissue Repair of Ministry of Education, Chongqing 400044, China.
Acta Biomater. 2009 Nov;5(9):3593-604. doi: 10.1016/j.actbio.2009.05.021. Epub 2009 May 27.
Plasma nanocoated films with trimethylsilane-oxygen monomers showed outstanding biocompatibility in our previous studies. In this study, endothelialization on biomedical nitinol alloy surfaces was systematically investigated. Our study focuses on elucidating the effects of surface micropatternings with micropores and microgrooves combined with plasma nanocoating. Plasma nanocoatings with controlled thickness between 40 and 50 nm were deposited onto micropatterned nitinol surface in a direct current plasma reactor. Bovine aortic endothelial cells were cultured in vitro on these nitinol samples for 1, 3 and 5 days. It was found that rougher surfaces could enhance cell adhesion compared with the smoother surfaces; the surfaces patterned with micropores showed much more endothelialization than microgrooved surface after a 3 days culture. The cell culture results also showed that plasma nanocoatings significantly further increased cell proliferation and cell adhesion on the micropatterned nitinol surfaces, as compared with non-plasma nanocoated surface of nitinol samples. The surface micropatternings combined with plasma nanocoatings could improve the cell adhesion and accelerate surface endothelialization after implantation of intravascular stents, which is expected to reduce in-stent restenosis.
在我们之前的研究中，带有三甲基硅烷-氧单体的等离子体纳米涂层薄膜表现出了出色的生物相容性。在这项研究中，我们系统地研究了生物医学钛镍诺合金表面的内皮化。我们的研究重点是阐明结合等离子体纳米涂层的微孔和微槽表面微图案化的效果。在直流等离子体反应器中，将厚度在 40 到 50nm 之间的等离子体纳米涂层沉积到微图案化的钛镍诺表面上。将这些钛镍诺样品在体外培养牛主动脉内皮细胞 1、3 和 5 天。结果发现，与更光滑的表面相比，较粗糙的表面可以增强细胞黏附；经过 3 天培养，微孔图案表面的内皮化程度明显高于微槽表面。细胞培养结果还表明，与钛镍诺样品的非等离子体纳米涂层表面相比，等离子体纳米涂层显著进一步促进了微图案化钛镍诺表面上的细胞增殖和细胞黏附。表面微图案化结合等离子体纳米涂层可以改善血管内支架植入后的细胞黏附，并加速表面内皮化，从而有望减少支架内再狭窄。
