a Central Laboratory, The Dongguan Affiliated Hospital of Medical College of Jinan University , The Fifth People's Hospital of Dongguan , Dongguan , China.
b Department of Cardiology, The Dongguan Affiliated Hospital of Medical College of Jinan University , The Fifth People's Hospital of Dongguan , Dongguan , China.
J Biomater Sci Polym Ed. 2018 Oct;29(15):1909-1919. doi: 10.1080/09205063.2018.1517858. Epub 2018 Oct 8.
As a promising candidate, biodegradable Poly-L-lactic Acid (PLLA) has been extensively used in coronary artery stents. In our previous reports, PLLA stents implanted in porcine coronary arteries showed safety without stent thrombosis. However, inflammatory responses were observed, which needed further study. In this study, human aortic endothelial cells (HAEC) were treated with different volume percentages of extract of pre-degraded PLLA (extract of PLLA) in vitro, and the cell growth curve and morphological changes were examined. The expression of inflammatory cytokines such as NF-κB, VEGF and VCAM-1 were also observed by ELISA. In addition, PLLA stent was implanted in porcine coronary artery to examine morphological changes, functional marker eNOS and inflammatory responses. The extract of PLLA caused significant growth inhibition and release of NF-κB, VEGF and VCAM-1 in HAEC with volume percentage-dependence. Although re-endothelialization and expression of eNOS was observed, expression of NF-κB and lymphocytes surrounding PLLA were also found after PLLA stents were implanted in the artery. This study demonstrated the effects of inflammation on endothelial cells induced by PLLA degradation in vitro and showed the inflammation in vivo, suggesting that anti-inflammatory strategy is necessary for PLLA stent implantation in the artery.
作为一种有前途的候选材料，可生物降解的聚左旋乳酸（PLLA）已被广泛用于冠状动脉支架。在我们之前的报告中，植入猪冠状动脉的 PLLA 支架显示出安全性，没有支架血栓形成。然而，观察到了炎症反应，需要进一步研究。在这项研究中，体外用人主动脉内皮细胞（HAEC）处理不同体积百分比的预降解 PLLA 提取物（PLLA 提取物），并检查细胞生长曲线和形态变化。还通过 ELISA 观察了炎症细胞因子如 NF-κB、VEGF 和 VCAM-1 的表达。此外，将 PLLA 支架植入猪冠状动脉，以检查形态变化、功能标志物 eNOS 和炎症反应。PLLA 提取物以体积百分比依赖性的方式引起 HAEC 显著的生长抑制和 NF-κB、VEGF 和 VCAM-1 的释放。尽管观察到再内皮化和 eNOS 的表达，但在动脉中植入 PLLA 支架后也发现了 NF-κB 和围绕 PLLA 的淋巴细胞。这项研究证明了 PLLA 降解在体外对内皮细胞的炎症作用，并显示了体内的炎症，表明在动脉中植入 PLLA 支架需要抗炎策略。
