弯曲杆菌多配体化学感受器（CcmL）传感结构域的克隆、重折叠、纯化及初步晶体学分析
Cloning, refolding, purification and preliminary crystallographic analysis of the sensory domain of the Campylobacter chemoreceptor for multiple ligands (CcmL).
作者信息
Machuca Mayra A, Liu Yu C, Beckham Simone A, Roujeinikova Anna
机构信息
Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia.
出版信息
Acta Crystallogr F Struct Biol Commun. 2015 Feb;71(Pt 2):211-6. doi: 10.1107/S2053230X1500045X. Epub 2015 Jan 28.
Abstract
A periplasmic sensory domain of the Campylobacter jejuni chemoreceptor for multiple ligands (CcmL) has been crystallized by the hanging-drop vapour-diffusion method using polyethylene glycol 3350 as a precipitating agent. A complete data set was collected to 1.3 Å resolution using cryocooling conditions and synchrotron radiation. The crystals belonged to space group P21, with unit-cell parameters a = 42.6, b = 138.0, c = 49.0 Å, β = 94.3°.
摘要
空肠弯曲杆菌多配体化学感受器（CcmL）的周质感觉结构域已通过悬滴气相扩散法，以聚乙二醇3350作为沉淀剂进行了结晶。使用低温冷却条件和同步辐射，收集了完整的数据集，分辨率达到1.3 Å。晶体属于P21空间群，晶胞参数为a = 42.6、b = 138.0、c = 49.0 Å，β = 94.3°。
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