Aydin Inci, Dimitropoulos Alexandra, Chen Shih Hsun, Thomas Connor, Roujeinikova Anna
Department of Microbiology, Monash University, Clayton, Victoria 3800, Australia.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 May 1;67(Pt 5):604-7. doi: 10.1107/S1744309111010219. Epub 2011 Apr 28.
Vibrio parahaemolyticus is a human pathogen associated with gastroenteritis caused by the ingestion of contaminated raw seafood. V. parahaemolyticus is able to survive exposure to low temperatures typical of those used for the refrigeration of foods by entering a viable but nonculturable (VBNC) state. The VBNC cells can regain culturability and renewed ability to cause infection upon temperature upshift. The resuscitation-promoting factor protein (Rpf, YeaZ) plays a key role in reactivation of growth. Crystals of V. parahaemolyticus YeaZ have been grown using the hanging-drop vapour-diffusion method with polyethylene glycol as a precipitating agent. The crystals belonged to the primitive monoclinic space group P2(1), with unit-cell parameters a = 81.7, b = 63.8, c = 82.3 Å, β = 105° and four subunits in the asymmetric unit. A complete X-ray diffraction data set was collected from a single crystal to 3.1 Å resolution.
副溶血性弧菌是一种人类病原体，与因摄入受污染的生海鲜而导致的肠胃炎有关。副溶血性弧菌能够通过进入活的但不可培养（VBNC）状态，在暴露于食品冷藏所采用的典型低温环境下存活。VBNC细胞在温度升高时可恢复可培养性并重新获得引发感染的能力。复苏促进因子蛋白（Rpf，YeaZ）在生长重新激活中起关键作用。副溶血性弧菌YeaZ的晶体已采用悬滴气相扩散法，以聚乙二醇作为沉淀剂生长而成。这些晶体属于原始单斜空间群P2(1)，晶胞参数a = 81.7、b = 63.8、c = 82.3 Å，β = 105°，不对称单元中有四个亚基。从一个单晶收集了完整的X射线衍射数据集，分辨率达到3.1 Å。
