通过电化学铂沉积提高扫描电化学显微镜-扫描离子电导显微镜的电化学成像灵敏度。

Improving the electrochemical imaging sensitivity of scanning electrochemical microscopy-scanning ion conductance microscopy by using electrochemical Pt deposition.

作者信息

Şen Mustafa, Takahashi Yasufumi, Matsumae Yoshiharu, Horiguchi Yoshiko, Kumatani Akichika, Ino Kosuke, Shiku Hitoshi, Matsue Tomokazu

机构信息

†Graduate School of Environmental Studies, Tohoku University, Sendai 980-8579, Japan.

§Advanced Institute for Materials Research (AIMR), Tohoku University, Sendai 980-8577, Japan.

出版信息

Anal Chem. 2015 Mar 17;87(6):3484-9. doi: 10.1021/acs.analchem.5b00027. Epub 2015 Feb 23.

DOI:10.1021/acs.analchem.5b00027

PMID:25665161

Abstract

We fabricated a platinum-based double barrel probe for scanning electrochemical microscopy-scanning ion conductance microscopy (SECM-SICM) by electrodepositing platinum onto the carbon nanoelectrode of the double barrel probe. The deposition conditions were optimized to attain highly sensitive electrochemical measurements and imaging. Simultaneous SECM-SICM imaging of electrochemical features and noncontact topography by using the optimized probe afforded high-resolution images of epidermal growth factor receptors (EGFR) on the membrane surface of the A431 cells.

摘要

我们通过将铂电沉积到双筒探针的碳纳米电极上，制备了一种用于扫描电化学显微镜-扫描离子电导显微镜（SECM-SICM）的铂基双筒探针。对沉积条件进行了优化，以实现高灵敏度的电化学测量和成像。使用优化后的探针同时进行电化学特征和非接触形貌的SECM-SICM成像，可获得A431细胞膜表面表皮生长因子受体（EGFR）的高分辨率图像。