Gossez Morgane, Poitevin-Later Francoise, Demaret Julie, Jallades Laurent, Venet Fabienne, Malcus Christophe, Monneret Guillaume
Immunology Laboratory, Hospices Civils De Lyon, Hôpital Edouard Herriot, Lyon, F 69003, France.
Hematology Laboratory, Hospices Civils De Lyon, Hôpital Edouard Herriot, Lyon, F 69003, France.
Cytometry B Clin Cytom. 2015 Nov-Dec;88(6):371-4. doi: 10.1002/cyto.b.21231. Epub 2015 Jul 16.
Pneumatic tube system (PTS) for transportation of blood specimens may present with advantages for hospital organization as it provides faster tube transfer from medical wards to routine labs. These characteristics are expected to result in faster sample processing and decreased turnaround time, therefore benefiting the patient particularly in emergency units. However, PTS could affect sample quality and therefore laboratory results. Within the context of routine lab certification, effects of PTS on routine cellular immunology analyses, especially on determination of T lymphocyte subpopulations, need to be evaluated.
Paired EDTA blood samples were collected from 30 healthy donors. For each pair, one sample was hand-delivered by a courier while the other was transported through a PTS of 2.4 km long (1.6 miles) with two 90-degree turns and one-U turn generating a speed of 5 m/s with a maximal acceleration of 2 g-force. The percentages of CD3+ cells, CD4+ and CD8+ T-cells and their absolute counts were assessed by flow cytometry.
For every parameter, results measured by flow cytometry were not significantly different after transport by PTS or hand-delivery. Results comparison revealed an excellent linear correlation between both delivery methods (R ranged from 0.969 to 0.982; P < 0.01). Bland-Altman plots also showed good agreement, indicating that results were not influenced by the transport method.
Our results suggest that, pending validation using clinical samples, PTS does not present with pre-analytical drawbacks and is thus a reliable system for blood samples transportation when performing T cell subset phenotyping.
用于运送血液标本的气动管道系统（PTS）对医院组织可能具有优势，因为它能更快地将标本从医疗病房转运至常规实验室。这些特性有望加快样本处理速度并缩短周转时间，从而尤其使急诊科室的患者受益。然而，PTS可能会影响样本质量，进而影响实验室结果。在常规实验室认证的背景下，需要评估PTS对常规细胞免疫学分析的影响，尤其是对T淋巴细胞亚群测定的影响。
从30名健康供者采集成对的乙二胺四乙酸（EDTA）抗凝血样本。对于每一对样本，一个由快递员人工送达，另一个通过一条2.4千米（1.6英里）长、有两个90度转弯和一个U形转弯的PTS进行运送，速度为5米/秒，最大加速度为2个重力加速度。通过流式细胞术评估CD3⁺细胞、CD4⁺和CD8⁺T细胞的百分比及其绝对计数。
对于每个参数，通过流式细胞术测得的结果在经PTS运送或人工送达后无显著差异。结果比较显示两种运送方式之间具有极好的线性相关性（R范围为0.969至0.982；P<0.01）。布兰德-奥特曼图也显示出良好的一致性，表明结果不受运送方式的影响。
我们的结果表明，在使用临床样本进行验证之前，PTS不存在分析前的缺陷，因此在进行T细胞亚群表型分析时是一种可靠的血液样本运送系统。
