Manolov A I, Dolgikh V V, Ukraintseva Iu V, Zavalko I M, Revishchin A V, Pavlova G V, Pronina T S, Ugriumov M V, Dorokhov V B, Koval'zon V M
Ross Fiziol Zh Im I M Sechenova. 2014 Nov;100(11):1252-60.
A group of mice with preliminary implanted (under general anesthesia) electrodes for cortical EEG and nuchal EMG was subjected to continuous baseline 24-hr video and digital polysomnographic recording with the 12/12 light/dark schedule, and then injected subcutaneously with 24 or 48 mg/kg of MPTP toxin or (the control group) saline. The recordings were continued for 2 weeks more. A significant increase in activity and the waking percentage as well as decrease in REM sleep and NREM sleep (tendency) during the dark period as compared to the baseline and control recordings was found. The effect was seen just on the 7th day following MPTP administration and became significant by the 14th day. The effect was more pronounced after 48 mg/kg injection than after 24. There were no changes during the light period. Morphological control revealed a 70% and 35% decreases in the amount of tyrosine hydroxylase positive neurons in substancia nigra/pars compacta after 48 and 24 mg/kg of MPTP, respectively, as compared to the saline group.
一组预先（在全身麻醉下）植入用于皮层脑电图和颈部肌电图电极的小鼠，按照12/12光/暗周期进行连续24小时的基线视频和数字多导睡眠图记录，然后皮下注射24或48毫克/千克的MPTP毒素或（对照组）生理盐水。记录再持续2周。与基线和对照记录相比，发现黑暗期的活动和清醒百分比显著增加，快速眼动睡眠和非快速眼动睡眠减少（有趋势）。该效应在给予MPTP后的第7天就可见，到第14天变得显著。48毫克/千克注射后的效应比24毫克/千克注射后更明显。光照期没有变化。形态学对照显示，与生理盐水组相比，48毫克/千克和24毫克/千克的MPTP给药后，黑质致密部酪氨酸羟化酶阳性神经元数量分别减少70%和35%。
