Freund Natalia T, Scheid Johannes F, Mouquet Hugo, Nussenzweig Michel C
Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
Laboratory of Humoral Response to Pathogens, Department of Immunology, Institut Pasteur, Paris 75015, France.
J Immunol Methods. 2015 Mar;418:61-5. doi: 10.1016/j.jim.2015.01.011. Epub 2015 Feb 7.
Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) have elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5' end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5' end of human Igλ.
抗HIV-1广谱中和抗体（bNAbs）的分离与鉴定揭示了新的表位和病毒易损位点。抗HIV-1 bNAbs在其可变区基因中通常表现出高水平的体细胞突变。这一特性可能会限制抗体的鉴定，因为突变后的抗体序列不再与基于种系抗体序列设计的引物互补。在此，我们报道了一组针对Igλ轻链的新引物，它们与前导序列的5'端对齐，对于扩增在人Igλ 5'端含有突变和缺失的抗体非常高效。
