Langmuir-Blodgett (LB)-based nanobiocrystallography at the frontiers of cancer proteomics.
作者信息
Pechkova Eugenia, Bragazzi Nicola Luigi, Fiordoro Stefano, Nicolini Claudio
机构信息
Nanoworld Institute, Fondazione EL.B.A. Nicolini (FEN), Pradalunga, Bergamo, Italy Laboratories of Biophysics and Nanobiotechnology (LBN), Department of Medical Science, University of Genova, Genova, Italy.
Nanoworld Institute, Fondazione EL.B.A. Nicolini (FEN), Pradalunga, Bergamo, Italy Laboratories of Biophysics and Nanobiotechnology (LBN), Department of Medical Science, University of Genova, Genova, Italy European Synchrotron Radiation Facility (ESRF), Grenoble, France
出版信息
Anticancer Res. 2015 Feb;35(2):827-34.
BACKGROUND/AIM: Langmuir-Blodgett (LB) films used as templates for crystallization lead to marked changes in protein stability and water dehydration, despite slight changes in protein atomic structure. Herein, we discuss the importance of LB-based nanocrystallography at the frontiers of cancer proteomics focusing on two model proteins with important biological roles in cancer, namely CK2alpha and RNase A.
MATERIALS AND METHODS
Computational mutagenesis using the KINARI Mutagen webserver exhibits different behaviors in terms of stability and robustness, as well as in terms of water dynamics.
CONCLUSION
Introduction of LB film leads to the appearance of water molecules close to the protein surface with larger volume, causing changes in crystal stability against radiation and appearing replicated in mutant proteins. Implications for drug design, drug delivery and cancer-causing protein variants are herein presented, along with a review of the most recent findings in LB-based nanobiocrystallography.
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