Avetisov S É, Sheremet N L, Muranov K O, Polianskiĭ N B, Polunin G S, Ostrovskiĭ M A
Eksp Klin Farmakol. 2014;77(11):11-5.
The effect of a mixture of N-acetylcarnosine and D-pantethine (1 : 1, m/m) on UV-A induced cataract in rats was studied. It is shown that instillation of a 5% mixture into the eyes or intraperitoneal injections (25 or 150 mg/kg) inhibit the formation of cataracts, starting from 82nd day of the experiment (p < 0.03), after which the protective effect of the mixture significantly increases (p = 0.0003). UV-A irradiation significantly (p < 0.01) increased the content of water-insoluble proteins in the lens. The use of the mixture of N-Acetylcarnosine and D-pantethine prevented (p < 0.001) an increase in the content of water-insoluble proteins caused by UV-A irradiation. Gel permeation chromatography data showed that, in the control group, water insoluble proteins consist of 3 fractions (40 kDa, 100 - 200 kDa, and1000 kDa). UV-A irradiation reduced the amount of protein in fraction 1 and increases the amount of protein in the fractions 2 and 3. The use of the mixture of N-acetylcarnosine and D-pantethine reduced the effects of UV-A light. The authors attribute the effect of the N-acetylcarnosine and D-pantethine mixture to their chaperone-like properties.
研究了N-乙酰肌肽和D-泛硫乙胺混合物（1:1，质量比）对紫外线A（UV-A）诱导的大鼠白内障的影响。结果表明，从实验第82天起，向大鼠眼内滴注5%的混合物或腹腔注射（25或150mg/kg）可抑制白内障的形成（p<0.03），之后混合物的保护作用显著增强（p = 0.0003）。UV-A照射显著（p<0.01）增加了晶状体中不溶性蛋白质的含量。使用N-乙酰肌肽和D-泛硫乙胺混合物可防止（p<0.001）UV-A照射引起的不溶性蛋白质含量增加。凝胶渗透色谱数据显示，在对照组中，不溶性蛋白质由3个组分组成（40kDa、100 - 200kDa和1000kDa）。UV-A照射减少了组分1中的蛋白质含量，增加了组分2和3中的蛋白质含量。使用N-乙酰肌肽和D-泛硫乙胺混合物可减轻UV-A光的影响。作者将N-乙酰肌肽和D-泛硫乙胺混合物的作用归因于它们类似伴侣蛋白的特性。
