Murray A W, Kirschner M W
Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143-0448.
Nature. 1989 May 25;339(6222):275-80. doi: 10.1038/339275a0.
We have produced extracts of frog eggs that can perform multiple cell cycles in vitro. Destruction of the endogenous messenger RNA arrests the extracts in interphase. The addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles. The newly synthesized cyclin protein accumulates during each interphase and is degraded at the end of each mitosis.
我们制备了能够在体外进行多个细胞周期的蛙卵提取物。内源性信使核糖核酸的破坏使提取物停滞在间期。添加外源性细胞周期蛋白信使核糖核酸足以产生多个细胞周期。新合成的细胞周期蛋白在每个间期积累，并在每次有丝分裂结束时降解。
