Murray A W, Solomon M J, Kirschner M W
Department of Biochemistry and Biophysics, School of Medicine, University of California, San Francisco 94143-0448.
Nature. 1989 May 25;339(6222):280-6. doi: 10.1038/339280a0.
We show that cyclin plays a pivotal role in the control of mitosis. A proteolysis-resistant mutant of cyclin prevents the inactivation of maturation promoting factor and the exit from mitosis both in vivo and in vitro. We have used a fractionated extract to study the activation of MPF by added cyclin protein.
我们发现细胞周期蛋白在有丝分裂的调控中起关键作用。一种抗蛋白水解的细胞周期蛋白突变体在体内和体外均能阻止成熟促进因子的失活以及有丝分裂的退出。我们使用了一种分级分离提取物来研究添加细胞周期蛋白对MPF的激活作用。
