Comis A, Hartwick R F, Howden M E
Division of Biological and Health Sciences, Deakin University, VIC, Australia.
Toxicon. 1989;27(4):439-47. doi: 10.1016/0041-0101(89)90206-7.
The stability of both the lethal and hemolytic activities of box jellyfish (Chironex fleckeri) tentacle extract was assessed after various extraction procedures. Both activities were higher when no buffers or water were used during the initial extraction. Also, when the extract was first filtered through a Sep-pak C18 cartridge, the residual lethal titre, after incubation for 24 hr at room temperature, was increased 16-fold and hemolysis was increased 2.6-fold. Evidence for proteolytic activity in the extract was also obtained and monitored by size exclusion HPLC.
在经过各种提取程序后,对箱形水母(Chironex fleckeri)触手提取物的致死活性和溶血活性的稳定性进行了评估。在初始提取过程中不使用缓冲液或水时,这两种活性都更高。此外,当提取物首先通过Sep-pak C18柱过滤时,在室温下孵育24小时后,残留致死效价增加了16倍,溶血增加了2.6倍。提取物中蛋白水解活性的证据也通过尺寸排阻高效液相色谱法获得并进行了监测。