Watanabe T, Takaue Y, Kawano Y, Koyama T, Huq M A, Shimokawa T, Ninomiya T, Aga Y, Inai T, Hino M
Department of Pediatrics, University of Tokushima, Japan.
Bone Marrow Transplant. 1989 May;4(3):261-5.
The levels of circulating hematopoietic progenitor cells were measured sequentially in eight children receiving chemotherapy for acute leukemia or neuroblastoma. Significant increases in the progenitor levels (up to 50-fold in CFU-GM numbers) were observed during post-chemotherapy cytopenia in all cases, but differences among individuals in the kinetics of recovery of less committed progenitors (CFU-mix) contrasted with the synchronized-mode of expansion of committed progenitors (CFU-GM). Peripheral blood cells were collected by repeated continuous-flow leukaphereses from three of the children during post-chemotherapy expansion of the progenitor pool and were cryopreserved after fractionation procedures. Infusion of these stored cells into the patients after marrow-ablative chemotherapy established trilineage hematopoiesis. This use of stem cell rescue should be useful as an alternative to bone marrow transplantation and extends the application of cure-oriented salvage therapy to childhood cancers.
对八名接受急性白血病或神经母细胞瘤化疗的儿童，依次测量了循环造血祖细胞水平。在所有病例中，化疗后血细胞减少期间均观察到祖细胞水平显著升高（CFU-GM数量增加高达50倍），但未定向祖细胞（CFU混合集落形成单位）恢复动力学的个体差异与定向祖细胞（CFU-GM）同步扩增模式形成对比。在祖细胞池化疗后扩增期间，通过重复连续流式白细胞分离术从三名儿童采集外周血细胞，并在分级分离程序后进行冷冻保存。在骨髓清除性化疗后将这些储存细胞输注给患者，实现了三系造血。这种干细胞救援的应用作为骨髓移植的替代方法应是有用的，并将以治愈为导向的挽救治疗应用扩展到儿童癌症。
