Zhai Jiao, Wang Yaling, Xu Chao, Zheng Lingna, Wang Meng, Feng Weiyue, Gao Liang, Zhao Lina, Liu Ru, Gao Fuping, Zhao Yuliang, Chai Zhifang, Gao Xueyun
Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences , Beijing, 100049, China.
Anal Chem. 2015 Mar 3;87(5):2546-9. doi: 10.1021/ac504639u. Epub 2015 Feb 13.
We report for the first time seeing and counting integrin α(IIb)β3 on a single-cell level. The proposed method is based on the using of the Au cluster probe. With the fluorescent property of Au24 cluster and the specific targeting ability of peptide, our probe can directly visualize integrin α(IIb)β3 on the membrane of human erythroleukemia cells (HEL) via confocal microscopy. On the basis of the accurate formula of our probe (Au24Peptide8), the number of integrin α(IIb)β3 can be precisely counted by quantifying the gold content on a single HEL cell via laser ablation inductively coupled plasma mass spectrometry. Our results reveal that the number of integrin α(IIb)β3 on a single cell varies from 5.75 × 10(-17) to 9.11 × 10(-17) mol, because of the heteroexpression levels of α(IIb)β3 on individual cells.
我们首次报告在单细胞水平上观察和计数整合素α（IIb）β3。所提出的方法基于金簇探针的使用。凭借Au24簇的荧光特性和肽的特异性靶向能力，我们的探针可通过共聚焦显微镜直接在人红白血病细胞（HEL）膜上可视化整合素α（IIb）β3。基于我们探针的精确配方（Au24Peptide8），通过激光烧蚀电感耦合等离子体质谱法对单个HEL细胞上的金含量进行定量，可精确计数整合素α（IIb）β3的数量。我们的结果表明，由于单个细胞上α（IIb）β3的异质表达水平，单个细胞上整合素α（IIb）β3的数量在5.75×10^(-17)至9.11×10^(-17)摩尔之间变化。
