在单纯疱疹病毒1型UL的异质片段中发现的串联重复序列。

A tandem repeat sequence found in a heterogeneous fragment of UL of herpes simplex virus type 1.

作者信息

Saito H, Sakaoka H, Yamashita T, Fujinaga K

机构信息

Department of Oral Bacteriology, School of Dentistry, Hokkaido University, Sapporo, Japan.

出版信息

J Gen Virol. 1989 Feb;70 ( Pt 2):443-8. doi: 10.1099/0022-1317-70-2-443.

DOI:10.1099/0022-1317-70-2-443

PMID:2567339

Abstract

We found a tandem repeat sequence in the region (designated BS7) in which restriction fragments BamHI D and SalI B overlap each other, near the centre of the unique long sequence (UL) of the herpes simplex virus type 1 (HSV-1) strain F genome. The SmaI physical map of BS7 was constructed, and the position of a heterogeneous SmaI subfragment from HSV-1 isolates and plaque-purified clones from a single strain was defined on the map. The maximum size difference in the SmaI subfragment was estimated to be 600 bp between these isolates and 100 bp between the clones. The 0.23 kb SmaI subfragment recloned from BS7 was sequenced, and was shown to contain a tandem repeat sequence consisting of 15 units of 12 bp, 5' TTGGGGCTGGGG 3'. These results suggest that the fragment length heterogeneity in the UL of HSV-1 isolates and clones is attributable to copy number variation of the tandem repeat sequence.

摘要

我们在1型单纯疱疹病毒（HSV-1）F株基因组独特长序列（UL）中心附近的区域（命名为BS7）发现了一个串联重复序列，其中BamHI D和SalI B限制性片段相互重叠。构建了BS7的SmaI物理图谱，并在该图谱上确定了来自HSV-1分离株和单个菌株的噬斑纯化克隆的异质SmaI亚片段的位置。这些分离株之间SmaI亚片段的最大大小差异估计为600 bp，克隆之间为100 bp。对从BS7中重新克隆的0.23 kb SmaI亚片段进行了测序，结果显示其包含一个由15个12 bp单元（5' TTGGGGCTGGGG 3'）组成的串联重复序列。这些结果表明，HSV-1分离株和克隆的UL中片段长度异质性归因于串联重复序列的拷贝数变异。